中文摘要：

[目的]获取GmHIR1相关生物信息学信息，分析与其他植物HIR的进化亲缘关系，进行启动子原件和结构的预测，分析其潜在调控功能，并研究该基因在疫霉菌侵染大豆过程中的表达情况。[方法]利用生物信息学软件对Gm—HIR1进行分析和功能预测，将大豆疫霉菌侵染抗病品种绥农10号和感病品种合丰25，分7个时间点进行取样，对Gm-HIR1的表达情况进行qRT—PCR分析。[结果]GmHIR1启动子含有11个作用元件，该基因编码蛋白含有stomatins和prohibitin两个结构域，表达分析表明GmHIR1在接种疫霉菌36h之前，在抗病品种绥农10号中表达明显增加，而在感病品种合丰25中变化不明显，36h之后，在两个品种的表达量变化趋势相似。[结论]GmHIR1与多种生理反应相关，并且确认GmHIR1与抗病大豆防御疫霉菌相关。

英文摘要：

[ Objective] In order to study the bioinformation of GmHIR1, including analysis of secondary structure and potential functions. Studied GmHIR1 function during Phytophthora sojae infecting soybean. [ Methods ] Function prediction and analysis of GmHIR1 using bioinformation software and websites. Suinongl0 and Hefeng25 was inoculated with Phytophthora sojae, then the stems were collected in 0 dpi,12 dpi,24 dpi,36 dpi,48 dpi,60 dpi and 72 dpi. qRT -PCR was used to detected the HIR gene transcript profile. [ Results ] GmHIR1 promoter has 11 elements which involved many important reactions, including abiotic stress,rhythm regulation, and microbial interactions, and the GmHIR1 has two important domains stomatins and prohibitin. Through the qRT- PCR results, we discovered the GmHIR1 was significant induced in Suinogl4, but not in Hefeng 25 before the 36 dpi. Then GmHIR1 was similarily induced in Suinongl4 and Hefeng 25 after the 36 dpi. [ Conclusion] We confirmed that GmHIR1 involved many regulation and it involved the process that soybean defeating the Phytophthora sojae.