中文摘要：

大黄 croaker， Larimichthys crocea 是在有高经济的价值的中国的一条重要的海洋的鱼。在最后十年，因为野人口倒塌和重要经济特点的退化，这种的股票保存和水产业工业一直在面对严重挑战。然而，在 L 的基因贡献生长和免疫。crocea 彻底地没被分析，并且可得到的分子的标记仍然不为基因资源管理和分子的选择是足够的。在这个工作，我们在 L 定序 transcriptome。有定序站台的 Roche 454 的 crocea 肝织物并且装配了 transcriptome 进 93 801 个抄本。他们， 38 856 个抄本成功地在 nt 被注解， nr， Swiss-Prot， InterPro，轮牙，去并且 KEGG 数据库。基于注解信息，与生长和免疫有关的 3 165 unigenes 被识别。另外， 6 391 次简单顺序重复(SSR ) 的一个总数从 transcriptome， 4 498 SSR 在之中有足够的 flanking 区域为聚合酶链反应(PCR ) 设计教材被识别。存取这些标记的多型性， 30 教材对随机在 30 个个人，和展出的 12 教材对(40.0%) 为 PCR 扩大和确认被选择明显的长度多型性。这个工作使用了 RNA-Seq 在 L 装配并且分析实时 transcriptome。crocea。与基因注解和顺序信息，与生长和免疫有关的基因被识别，巨大的 SSR 标记被开发，提供为未来基因的珍贵基因资源功能的分析并且 L 的选择繁殖。crocea。

英文摘要：

The large yellow croaker, Larimichthys crocea is an important marine fish in China with a high economic value. In the last decade, the stock conservation and aquaculture industry of this species have been facing severe challenges because of wild population collapse and degeneration of important economic traits. However, genes contributing to growth and immunity in L. crocea have not been thoroughly analyzed, and available molecular markers are still not sufficient for genetic resource management and molecular selection. In this work, we sequenced the transcriptome in L. eroeea liver tissue with a Roche 454 sequencing platform and assembled the transcriptome into 93 801 transcripts. Of them, 38 856 transcripts were successfully annotated in nt, nr, Swiss-Prot, InterPro, COG, GO and KEGG databases. Based on the annotation information, 3 165 unigenes related to growth and immunity were identified. Additionally, a total of 6 391 simple sequence repeats （SSRs） were identified from the transcriptome, among which 4 498 SSRs had enough flanking regions to design primers for polymerase chain reactions （PCR）. To access the polymorphism of these markers, 30 primer pairs were randomly selected for PCR amplification and validation in 30 individuals, and 12 primer pairs （40.0%） exhibited obvious length polymorphisms. This work applied RNA-Seq to assemble and analyze a live transeriptome in L. crocea. With gene annotation and sequence information, genes related to growth and immunity were identified and massive SSR markers were developed, providing valuable genetic resources for future gene functional analysis and selective breeding ofL. eroeea.