中文摘要：

利用Illumina HiSeqTM 2500测序平台, 对通过高温胁迫实验筛选得到的20尾耐高温和20尾不耐高温的大黄鱼（Larimichthys crocea）进行了简化基因组测序（SLAF-seq）, 每个样本的平均测序深度达到10.26×, 共获得419211个高质量的群体单核苷酸多态性（SNP）位点 .利用TASSEL软件的混合线性模型（MLM）进行全基因组关联分析（GWAS）, 共筛选到38个与大黄鱼耐高温性状显著相关的SNP位点（P〈2.39E–08）.利用BLAST程序定位每个SNP位点在大黄鱼基因组中的位置, 并分析其周围的功能基因.结果在38个SNPs附近共找到26个已知的功能基因, 这些基因主要与细胞转录、代谢、免疫等功能相关.研究结果可为下-步大黄鱼耐高温分子机制解析及耐高温品种的选育提供参考.

英文摘要：

Twenty thermal-tolerant and twenty thermal-sensitive individuals of Larimichthys crocea were sequencedusing specific-locus amplified fragment （SLAF-seq） technology based on Illumina HiSeqTM2500 platform. 419211 SN-Ps were identified with an average read depth of 10.26× for each sample. Thirty-eight SNPs （P〈2.39E–08） signifi-cantly related with thermal tolerance trait were identified according to association analysis. The SNP locations in largeyellow croaker genome were identified using BLAST program, and functional genes around SNP were annotated.Twenty-six genes with known functions were discovered around 38 SNPs, which mainly regulate cell transcription,metabolism and immunity. These results provide basic information to analyze thermal-tolerant molecular mechanismand develop thermal-tolerant lines of Larimichthys crocea in the future.