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大黄鱼快长相关微卫星标记的筛选与验证
  • ISSN号:1000-3207
  • 期刊名称:《水生生物学报》
  • 时间:0
  • 分类:Q346.5[生物学—遗传学] S965.3[农业科学—水产养殖;农业科学—水产科学]
  • 作者机构:[1]集美大学水产学院,农业部东海海水健康养殖重点实验室,厦门361021
  • 相关基金:国家863计划项目(2012AAl0A403);国家自然科学基金(31172397,31272653);福建省高校水产动物遗传育种科技创新团队项目资助
中文摘要:

为了筛选与大黄鱼生长性状紧密相关的分子标记,并对这些标记的有效性进行鉴定,研究以大黄鱼家系和群体为材料,经标记筛选、两次验证共三个步骤,找到2个与大黄鱼生长性状紧密相关的微卫星标记:LYC0088和LYC0143,其中LYC0088与体高、体质量均极显著相关(P〈0.01),与体长显著相关(P〈0.05),LYC0143与体长和体质量显著相关(P〈0.05),与体高的相关性极显著(P〈0.01)。LYC0088与LYC0143位点的优势等位基因分别为E、A,优势等位基因型均为AB,优势等位基因型组合为AB/AB,两个位点呈加性效应。该结果为开展大黄鱼分子标记辅助育种提供了有价值的遗传标记。

英文摘要:

To screen the molecular markers closely related with growth traits and identify the validity of these markers in large yellow croaker, a family and a population were used as material and three steps were included. The first step was makers screening. In this step, a family with 60 extreme individuals was used as genetic material. The second step was markers pre- liminary verification. In this step, a family with 120 random individuals was used as genetic material. The last step was markers verification once again. A population with 60 extreme individuals was used. Two microsatellite markers closely related with growth traits were obtained. LYC0088 was significantly correlated with body height, body weight (P〈0.01), and body length (P〈0.05). LYC0143 was significantly correlated with body length and body weight (P〈0.05), and body height (P〈0.01). For LYC0088 and LYC0143 loci, the advantage allele was E and A, respectively. The advantage genotypes were both AB. The advantage combination of genotype was AB/AB. These two loci presented additive effects. The results provide valuable ge- netic markers for conducting large yellow croaker molecular marker-assisted breeding.

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期刊信息
  • 《水生生物学报》
  • 北大核心期刊(2011版)
  • 主管单位:中国科学院
  • 主办单位:中科院水生所
  • 主编:桂建芳
  • 地址:武昌东湖南路7号中科院水生所
  • 邮编:430072
  • 邮箱:Acta@ihb.ac.cn
  • 电话:027-68780701
  • 国际标准刊号:ISSN:1000-3207
  • 国内统一刊号:ISSN:42-1230/Q
  • 邮发代号:82-329
  • 获奖情况:
  • 湖北省十佳重点期刊,中国农学会中国水产学会优秀科技期刊一等奖,中国科学院优秀期刊三等奖,中国期刊方阵“双效”期刊
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,美国生物科学数据库,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:21674