中文摘要：

目的：观察养血解毒汤对咪喹莫特诱导的STAT3转基因小鼠银屑病样皮损的干预作用。方法：STAT3转基因小鼠24只,随机分为正常对照组、模型组、养血解毒汤组和甲氨蝶呤组,每组6只,采用外用咪喹莫特诱导皮肤银屑病样模型。分别观察皮损面积和疾病严重程度（psoriasis area and severity index,PASI）,光镜下观察皮损组织形态学变化和表皮层厚度;采用皮肤水分油分测试笔检测小鼠背部皮肤水分和油分含量;免疫组织化学法检测皮损中增殖细胞核抗原（proliferating cell nuclear antigen,PCNA）和T淋巴细胞表面标志CD3表达;采用实时荧光定量PCR技术检测皮损Th17类细胞因子（IL-17A、IL-17C、IL-22和RORγt）mRNA表达;采用Western blot检测皮损STAT3通路蛋白（STAT3、p-STAT3、JAK3、p-JAK3和SOCS3）的表达水平。结果：（1）养血解毒汤组PASI评分明显低于模型组,HE染色观察发现养血解毒汤组皮损表皮增生较模型组少,角化不全的细胞明显减少,皮肤厚度降低（P〈0.01）;（2）养血解毒汤组皮损油分和水分含量均明显高于模型组（P〈0.05）;（3）养血解毒汤组PCNA阳性表达远低于模型组（P〈0.01）;（4）养血解毒汤组脾脏重量低于模型组（P〈0.05）;（5）养血解毒汤组Th17相关因子（IL-17A、IL-17C、IL-22和RORγt）表达水平均低于模型组,此外,p-STAT3和p-JAK3表达量亦低于模型组,而SOCS3的表达高于模型组。结论：养血解毒汤可能通过抑制STAT3的磷酸化,减少Th17相关因子（IL-17A、IL-17C、IL-22和RORγt）的分泌,由此改善咪喹莫特诱导的STAT3转基因小鼠银屑病样皮损改变。

英文摘要：

AIM： To observe the effects of Yangxue-Jiedu（ YXJD） decoction on imiquimod-induced psoriasislike lesions in STAT3 transgenic mice. METHODS： STAT3 transgenic mice（ n = 24） were randomly divided into 4groups： control group（ using purified water for oral administration）,model group（ topical 5% imiquimod 42 mg and using purified water for oral administration）,YXJD groups（ topical 5% imiquimod 42 mg and using YXJD decoction for oral administration）,and methotrexate（ MTX） group（ 1 mg / kg MTX solution for oral administration,with the same topical imiquimod as model group）. On day 7,the skin lesions were collected for examination. The lesions were evaluated according to the psoriasis area and severity index（ PASI）. The skin barrier function was evaluated by assessing oil and water components in the skin. The inflammation of psoriasis-like lesions was assessed by histological method. The expression of proliferating cell nuclear antigen（ PCNA） and CD3 was assessed by immunohistochemical staining. The mRNA expression of IL-17 A,IL-17 C,IL-22 and RORγt was detected by real-time PCR. The levels of JAK / STAT3 pathway-related proteins in isolated T cells were determined by Western blot. RESULTS： Administration of YXJD decoction inhibited imiquimod-induced keratinocyte proliferation and infiltration of CD3＋T cells in psoriatic lesions,and ameliorated the epidermal barrier by up-regulation of the oil and water components in psoriatic lesions. Meanwhile,administration of YXJD decoction improved the systemic immune responses by reducing the weight of the spleen. The inflammatory cytokines IL-17 A,IL-17 C,IL-22 and RoRγt,and the levels of JAK / STAT3 pathway-related proteins STAT3,p-STAT3,JAK3 and p-JAK3 were decreased by administration of YXJD decoction. CONCLUSION： YXJD decoction likely alleviates imiquimod-induced psoriasis-like lesions in the STAT3 transgenic mice by inhibiting the phosphorylation of STAT3,and reducing the expression of IL-17 A,IL-17 C,IL-22 and RORγt.