以"陇油9号"白菜型油菜幼苗为试材,利用低温培养箱模拟低温条件,通过叶面喷施复合外源物质,研究了复合外源物质对白菜型油菜幼苗抗寒性的影响。结果表明:以抗寒性植物水提液为基液配置的复合外源物质处理可使油菜幼苗在低温(0±1)℃胁迫下保持相对较高的超氧化物歧化酶(SOD)活性和葡萄糖、可溶性蛋白质、叶绿素、脯氨酸(Pro)含量,减少丙二醛(MDA)含量的积累,提高了"陇油9号"白菜型油菜幼苗的抗寒性;复合外源物质可在一定程度上提高"陇油9号"白菜型油菜幼苗的抗寒能力,有效降低"陇油9号"白菜型油菜幼苗的冻害。
The Brassica campetris(rape)‘Longyou 9'seedling was selected as experimental material,and the low temperature conditions were stimulated with low temperature incubator.The Brassica campetris(rape)‘Longyou 9'seedling were treated with compound biological preparation as foliar application to study the effect of low temperature stress on it.The results showed that the composite exogenous substances of cold resistance of plant water solution for the base configuration treatment could make the rape seedlings under the stress of low temperature(0±1)℃ maintain a relatively high superoxide dismutase(SOD)activity and the contents of glucose,soluble protein,chlorophyll and proline(Pro),reduce the malondialdehyde(MDA)accumulation,improve the cold resistance of Brassica campetris(rape)‘Longyou 9'.Composite exogenous substances could increase the Brassica campetris(rape)‘Longyou 9'seedlings fight cold ability,effectively reduce Brassica campetris(rape)‘Longyou 9'seedling frost damage.