中文摘要：

目的：研究单核／巨噬细胞分化成为泡沫细胞过程中ghrelin对人A11P结合盒转运子A1和G1（ABCA1／ABCG1）表达的调控作用。方法：体外培养人源单核细胞系THP-1，由佛波酯（PMA）作用使其分化为巨噬细胞，后者可在氧化低密度脂蛋白（OX—LDL）存在条件下进一步转变为泡沫细胞。巨噬细胞加入不同浓度的Ghrelin预孵2h后再加入OX—LDL作用不同时间，油红O染色法观察细胞内脂滴含量，运用PT—PCR法检测ABCA1和ABCG1mRNA水平，Western blotting法检测ABCA1和ABCG1蛋白表达，采用酶法，通过荧光分光光度计检测细胞内外胆固醇含量并计算胆固醇流出率。结果：Ghrelin干预可明显减少细胞内脂滴的形成，显著增加胆固醇流出率，并能显著增加单核／巨噬细胞泡沫化过程中ABCA1和ABCG1mRNA水平和蛋白表达，且此作用呈浓度依赖性而不呈时间依赖性。结论：Ghrelin可能通过上调ABCA1和ABCG1转录翻译水平延缓动脉粥样硬化的发生。

英文摘要：

AIM ： To investigate the regulation of ghrelin on the expression of ATP - binding cassette transporter A1 and G1 （ABCA1/ABCG1）during the foam cell formation. METHODS： The human monocytie leukemia cell line （THP - 1 ） was chosen in our study. The differentiation of THP - 1 cells into macrophages was induced by using phorbol myristate acetate （PMA）. Macrophages were then incubated with oxidized LDL （ox -LDL）to generate foam cells. Ghrelin of different concentrations were treated at different time points during foam cell formation. The ABCA1/ABCG1 protein and mRNA levels were detected by Western blotting and RT - PCR. The effect of variance of cholesterol content was measured by zymochemistry via - fluorospectrophotometer. RESULTS ： Ghrelin reduced the content of lipid droplet in foam cells, and increased the efflux of intracellular cholesterol significantly. Ghrelin increased ABCA1 protein mass and mRNA level in dose - dependent manner. The changes of ABCG1 protein and mRNA level were the same as ABCA1. CONCLUSION： Ghrelin interfere atherosclerosis by up - regulating the expression of ABCA1 and ABCG1.