中文摘要：

应用PCR方法从人单核细胞系THP-1基因组DNA扩增人酰基辅酶A：胆固醇酰基转移酶1（acyl coenzyme A：cholesterol acyltransferasel，ACAT1）基因P7启动子全长片段，进行TA克隆，经双酶切、测序鉴定阳性克隆。将该基因亚克隆至荧光素酶报告基因载体pGL3-Enhancer，用DEAE-葡聚糖（DEAE-dextm）法将重组质粒pGL3E-P7瞬时转染入单核细胞THP-1，检测其活性，证明pGL3E-P7能在体外表达荧光素酶。人ACAT1基因P7启动子调控的荧光素酶报告基因表达载体的成功构建，为研究动脉粥样硬化过程中ACAT1基因的转录调控机制提供新手段。

英文摘要：

The DNA segment of the human acyl coenzyme A： cholesterol acyltransferasel （ACAT1） gene P7 promoter was amplified by PCR from human monocytic leukemia cell line （THP-1） and cloned to TA vector, then the positive clone was confirmed by restriction enzymes and sequencing. The targeted segment was subcloned to Firefly luclferase report vector pOL3-Enhancer. The recombinant plasmid pGL3E-P7 was transfected transiently into THP-1, then the expression of luciferase could be detected in THP-1 by pGL3E-P7 transfection. We successfully constructed luciferase reporter vector containing P7 promoter of the human ACAT1 gene, and established a new means to study the transcriptional regulation mechanisms of ACAT1 during atherosclerosis.