中文摘要：

目的克隆人酰基辅酶A：胆固醇酰基转移酶1（ACAT1）基因P1启动子。方法应用PCR方法从人单核细胞系THP-1扩增分离出ACAT1基因P1启动子全长片段．将PCR产物克隆入T载体，并对所获得的序列进行生物信息学分析。结果经琼脂糖凝胶电泳及直接测序鉴定，克隆的ACAT1基因P1启动子片段碱基序列与GenBank数据库一致，未发现突变。结论人ACAT1基因P1启动子克隆成功，此为动脉粥样硬化（AS）过程中ACAT1基因转录调控机制的研究奠定了基础。

英文摘要：

[Objective] To clone P1 promoter of the human acyl eoenzyme A： cholesterol acyltransferasel1 （ACAT1） gene. [Methods] The complete nucleotide segment of P1 promoter was obtained from human monocytic leukemia cell line （THP-1） by PCR method. The PCR product was inserted into pMD19-T simple vector, and the sequence was analyzed by biological information method. [Results] The DNA sequence of P1 promoter cloned was accordant with GenBank data, and no mutation was found. [Conclusions] P1 promoter of the ACAT1 gene can be cloned successfully. These results may make an important basis for studying transcriptional regulation mechanisms of ACAT1 during atherosclerosis.