中文摘要：

膜蛋白质在房间是关键的生理的活动并且是为大多数药的目标。因此，调查膜蛋白质的行为不仅提供更深的卓见进房间功能，而且帮助疾病处理和药开发。原子力量显微镜学是为调查膜蛋白质的结构的一个唯一的工具。它能两个都想象形态学与高分辨率挑选本国的膜蛋白质并且经由单个分子的力量光谱学(SMFS ) ，直接在象展开的 ligand 绑定和蛋白质那样的分子的生理的活动期间测量他们的生物物理的性质。在分子的 biomechanics 的上下文， SMFS 成功地被用来理解膜蛋白质的结构和功能，补充 X 光检查晶体学获得的蛋白质的静态的三维的结构。基于在临床的肿瘤房间的作者抗原抗体绑定力量大小，这里， SMFS 的原则和方法被讨论，在使用 SMFS 描绘膜蛋白质的进步被总结，并且为 SMFS 的挑战被提出。

英文摘要：

Membrane proteins are crucial in cell physiological activities and are the targets for most drugs. Thus, investigating the behaviors of membrane proteins not only provide deeper insights into cell function, but also help disease treatment and drug development. Atomic force microscopy is a unique tool for investigating the structure of membrane proteins. It can both image the morphology of single native membrane proteins with high resolution and, via singlemolecule force spectroscopy （SMFS）, directly measure their biophysical properties during molecular physiological activities such as ligand binding and protein unfolding. In the context of molecular biomechanics, SMFS has been successfully used to understand the structure and function of membrane proteins, complementing the static three-dimensional structures of proteins obtained by X-ray crystallography. Here, based on the authors＇ antigenantibody binding force measurements in clinical tumor cells, the principle and method of SMFS is discussed, the progress in using SMFS to characterize membrane proteins is summarized, and challenges for SMFS are presented.