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中文摘要：
单个病毒的三维操作对自下而上开展单分子尺度的细胞免疫学研究具有重要的意义,然而受操作工具及操作方法的限制,该问题一直未能得到很好的解决.以基于原子力显微镜的纳米操作机器人为基础,开展了针对单个病毒分子的三维操作研究,具体包括：高均匀分散性病毒样品制备方法,基于局部扫描的病毒三维操作结果实时检测策略,以及微观尺度下病毒三维空间的定点放置实现.实验结果表明,本文提出的操作方法与技巧,不仅能够实现单个病毒分子在三维空间内的可控定点拾取与释放,还可与二维操作方法相结合,构建出全病毒分子的三维纳米结构,实验结果验证了所提方法的正确性和有效性,从而为自下而上的细胞免疫学开展提供了可行的技术途径.
英文摘要：
The 3D manipulation of single virus has great significance to the researches of molecular and cellular immunology in a bottom-up way.However,this 3D manipulation has not been well demonstrated due to the limitations of operation tools and methods.In this paper,we carried out a detailed study of controllable single virus 3D manipulation based on the nanorobot developed from the Atomic Force Microscope（AFM）,it mainly concludes the method of preparing high uniform and scattered virus sample,the real-time sensing strategy of 3D manipulation results based on local scan technique,and the implementation skills of releasing single virus in a certain location controllably.The experimental results show that single virus can not only be controllably picked up or released in certain place,but can be constructed into 3D structure successfully with the method we proposed,which verifies the validity and effectiveness of the proposed method,and further provides a feasible way for the study of bottom-up cellular immunology.
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