中文摘要：

原子力量显微镜学(AFM ) 被用来在淋巴瘤 Raji 房间的表面上定位 CD20 分子。Rituximab (对 CD20 的 monoclonal 抗体) 分子经由聚乙烯乙二醇(木钉) 被连接到 AFM 尖端上连接器。Raji 房间被吸附到与 poly-L-lysine 涂的玻璃幻灯片上。首先，在房间表面的一个本地区域的 CD20 分发用 AFM 电梯扫描模式被设想。第二， 16

英文摘要：

Atomic force microscopy （AFM） was used to locate CD20 molecules on the surface of lymphoma Raji cells. Rituximab （a mono- clonal antibody against CD20） molecules were linked onto the AFM tip via a polyethylene glycol （PEG） linker. Raji cells were adsorbed onto glass slides coated with poly-L-lysine. First, the CD20 distribution in a local area of the cell surface was visualized using the AFM lift scan mode. Second, 16 x 16 force curves were obtained from the same cell area to construct the CD20- rituximab binding force map. Finally, free rituximab was added to block the CD20 molecules on the cell surface and the lift phase image and CD20-rituximab force map were obtained again. The experimental results indicated that when the lift height was great- er than the length of the PEG linker, no recognition sites were observed in the lift phase image. However, as the lift height de- creased to the length of the PEG linker, some recognition sites were observed in the lift phase image and these sites were general- ly consistent with the pixels in the force map. After blocking, both the recognition sites in the lift phase image and the gray pixels in the binding force map decreased markedly. These results can improve our understanding of the distribution of protein mole- cules on the cell surface and facilitate further investigations into cellular functions.