中文摘要：

为克服采用传统方法分离木脂素的不足，采用高速逆流色谱法对珠子草中的木脂素进行分离，得到3个木脂素，通过红外光谱（ IR）、质谱（ MS）、核磁共振（ NMR）等方法鉴定为珠子草素（ niranthin）、叶下珠次素（hypophyllanthin）和叶下珠脂素（phyllanthin）。分离溶剂体系为正己烷/乙酸乙酯/甲醇/水（3/2/3/2，V/V），下相为流动相，流速为2 mL/min，转速为正转800 r/min，温度为25℃。实验结果为木脂素的分离提供了一条有效的途径。

英文摘要：

To overcome disadvantages of traditional lignans isolation methods,high-speed countercur-rent chromatography （ HSCCC） was employed and three lignans were isolated from Phyllanthus niru-ri Linn,which were identified as niranthin,hypophyllanthin,and phyllanthin by spectroscopic meth-ods,including infrared spectroscopy（IR）,mass spectrometry（MS）and nuclear magnetic resonance （ NMR） .Separating processes were given as following：light phase as stationary phase and heavy phase as mobile phase from mixture of n-hexane,ethyl acetate,methanol and water（3/2/3/2,V/V） with flow rate 2 mL/min,rotation speed 800 r/min at 25℃.The results provide an effective way for isolation of lignans.