中文摘要：

目的：研究愤怒应激对D-半乳糖（D-gal）诱导的脑老化模型大鼠认知功能的影响及机制。方法：将Wistar大鼠随机分成正常对照组、愤怒模型组、D-gal模型组愤怒＋D-gal模型组及攻击鼠,颈后部sc 1%D-gal生理盐水溶液,注射容量为10 mL.kg-1.d-1,正常对照组注射同体积生理盐水,连续注射6周。愤怒组、愤怒D-gal组,从第三周始进行愤怒刺激实验,把愤怒模型组大鼠单独放入鼠笼中,随机放进一只入侵鼠,用纱布包裹止血钳钳夹入侵鼠尾,入侵鼠对模型组大鼠发起攻击,双方互相撕咬对峙,每天刺激时间为20 min,连续4周。第37天开始Morris水迷宫实验测试,6 d后处死解剖大鼠,收集脑组织进行超氧化物歧化酶（SOD）、丙二醛（MDA）、脂褐素（LPF）检测;同时对大脑皮质及海马区进行病理学观察。结果：与正常对照组相比,愤怒模型组、D-gal模型组、愤怒＋D-gal模型组脑SOD活性明显降低（P〈0.01）,MDA,LPF含量明显升高（P〈0.01）,空间学习记忆能力明显降低（P〈0.05）;行为活动和学习记忆功能减退差异显著（P〈0.05,P〈0.01）;病理学检测结果显示,与正常对照组相比,愤怒模型组、D-gal模型组、愤怒＋D-gal模型组大脑皮质和海马区均出现不同程度的病理变化,其中愤怒＋D-gal模型组病变最为明显。结论：愤怒应激对D-gal诱导的脑老化模型大鼠的行为活动和认知功能具有减退作用。

英文摘要：

Objective：To investigate the effects and mechanism of anger stress on behavior and memory in the D-gal induced brain aging model rats.Method：Wistar rats were randomly divided into control group,anger model group,D-gal group,anger＋D-gal group,and attack rats.The D-galgroup and anger＋D-gal group were administered ip 1% D-gal physiological salinesolution（PSS） 10 mL · kg-1 · d-1.The control group and anger model group were ip given the same volume of PSS for 6 weeks.From the third weeks,the anger model group and anger＋D-gal group accepted the anger stimulation test for 4 weeks.Each rat in anger model group was placed into one cage and accepted the attack from the invade rat,the tail of invade rat was clamped by using haemostat packed with bandage,two rats bite each other 20 min per day.Morris water maze test was carried out in the 37 days.Six days later all rats were executed and the brain tissue was collected for measurement of superoxide dismutase（SOD）,malondialdehyde（MDA）,lipofuscin（LPF）,meanwhile,pathological changes were observed on cerebral cortex and seahorse.Result：Compared with control group,the behavior and memory significantly decreased（P0.05,P0.01）,the brain tissue SOD activity wassignificantly reduced（P0.01） and MDA,LPF content were obviously increased（P0.01） in the anger model group,D-gal group and anger＋D-gal group.Furthermore,pathological study showed that the cerebral cortex and seahorsein in the anger model group,D-gal group and anger＋D-gal group appeared pathological changes compared with control group,the pathological changes in anger＋D-gal group were most obvious.Conclusion：The anger stress has the negatively effects on behavior and memory in the D-gal induced brain aging model rats.