中文摘要：

PNRC（proline—rich nuclear receptor coactivator）是最近发现的一种新的核受体辅活化子。它是以牛类固醇生成因子-1（SF1）作诱饵，利用酵母双杂系统从人乳腺cDNA表达文库中筛选得到的．为了研究人PNRC基因的表达调控机制，在对人PNRC基因的生物信息分析的基础上。采用5’RACE法确定了PNRC基因的转录起始位点，克隆了人PNRC基因的5’侧翼区，并对该区进行功能分析．分别构建了11种含不同长度启动子序列的荧光素酶报告基因表达质粒，将它们瞬时转染HepG2细胞，检测其荧光素酶活性．结果发现，PNRC的5’侧翼区的-323-＋27、-323-＋57、-123-＋57、-123-＋27区域的启动子活性显著升高，其中-323-＋27区域的启动子活性最高．研究表明。人PNRC基因启动子的最小区域位于PNRC的5’侧翼区的-123-＋27区域．

英文摘要：

PNRC （proline-rich nuclear receptor coactivator）, a new nuclear receptor coactivator, was identified as bovine SF-1 （steroidogenie factor 1 ）-interacting proteins in a yeast two-hybrid screening of a human manunary gland eDNA expression library. To study the molecular mechanisms that regulate the expression of PNRC gene, we determined the potential transcriptional start sites of PNRC gene by 5＇RACE, then cloned the 5＇-flanking region of the gene. After analysed the 5＇-flanking region of PNRC, we constructed eleven luciferase expression vectors which contained human PNRC gene promoter. All expression vectors were transfected into HepG2 cell. The luciferase assays revealed that the - 123 - ＋ 27 region was the minimal promoter of the human PNRC gene.