中文摘要：

采用戊二醛（GA）一步法将半抗原伏马菌素B1（FB1）分别与鸡卵清蛋白（OVA）、牛血清白蛋白（BSA）偶联,制备2种具有免疫原性的FB1人工抗原FB1-OVA和FB1-BSA。分别采用凝胶电泳法、紫外光谱法（UV）、红外光谱法（IR）、基质辅助激光分析电离飞行时间质谱法（MALDI-TOF-MS）4种方法鉴定人工抗原偶联效果并测定偶联比。将FB1-BSA作为免疫抗原免疫BALB/c小鼠,FB1-OVA作为固相包被抗原,采用间接酶联免疫吸附法（i-ELISA）测定小鼠抗血清滴度。结果表明：4种方法均鉴定FB1人工抗原合成成功。凝胶电泳法测定FB1-OVA和FB1-BSA相对分子质量分别约为50 000和75 000。MALDI-TOF-MS测定FB1-OVA和FB1-BSA相对分子质量分别为48 009.212和74 355.301,并精确计算FB1-OVA和FB1-BSA偶联比分别为5∶1和10∶1。FB1-BSA免疫后小鼠的抗血清效价为1∶1.25×105,并具有较高灵敏度。

英文摘要：

In the study,the hapten fumonisin B1（FB1）was coupled with carrier proteins ovalbumin（OVA）and bovine serum albumin（BSA）respectively,by one step method with glutaraldehyde（GA）method,and two artificial antigens FB1-OVA and FB1-BSA were synthesized.Four methods of gel electrophoresis,ultraviolet spectroscopy（UV）,infrared spectroscopy（IR）and matrix-assisted laser absorption ionization time-of-flight mass spectrometry（MALDI-TOF-MS）were used to determine the coupled effects and coupling ratios of the artificial antigens.Then FB1-BSA was used to immunize BALB/c mice,and FB1-OVA was used as solid phase coating antigen.The indirect enzyme-linked immunosorbent assay（i-ELISA）was used to determine the titer of anti-serum from mice.The results showed that four methods all proved that carrier proteins coupled with FB1 successfully.The molecular weights of FB1-OVA and FB1-BSA were about 50 000 and 75 000 respectively by gel electrophoresis.The accurate molecular weights of FB1-OVA and FB1-BSA were 48 009.212 and 74 355.301 and the coupling ratios of FB1-OVA and FB1-BSA were 5∶1 and 10∶1 respectively by MALDI-TOF-MS.The titer of anti-serum was 1∶1.25×105 by i-ELISA with good sensitivity.