中文摘要：

[目的]本试验旨在研究脱氧血腐镰刀菌烯醇（deoxynivalenol,DON）对脂多糖（lipopolysaccharide,LPS）刺激的小鼠骨髓源性成熟树突状细胞（mature dendritic cell,m DC）的表型和吞噬功能的影响。[方法]体外培养小鼠骨髓细胞诱导分化成未成熟的树突状细胞,分别用不同质量浓度的DON（0、125、250、500 ng·mL^-1）处理DC 6 h后,再加入LPS刺激18 h。以仅用LPS处理的为阳性对照组。用流式细胞仪检测DC表型（CD80、CD86和MHC-Ⅱ）变化及吞噬右旋葡聚糖的能力;RTPCR检测细胞中细胞因子（IL-6、IL-0、IL-12和TNF-α）mRNA的相对表达。[结果]LPS刺激引起m DC表面的CD80、CD86和MHC-Ⅱ升高（P〈0.05）,吞噬右旋葡聚糖能力下降,IL-6、IL-0、IL-12和TNF-α的mRNA相对表达水平提高（P〈0.05）;低浓度和中浓度DON（125和250 ng·mL^-1）对DC的表型和免疫功能没有影响（P〉0.05）,高浓度DON（500 ng·mL^-1）引起m DC共刺激分子（CD80、CD86和MHC-Ⅱ）表达水平降低（P〈0.05）,同时细胞因子的mRNA表达降低（P〈0.05）,但吞噬能力提高12.2%。[结论]高浓度DON对LPS刺激的m DC表型和细胞因子表达具有一定的抑制作用。

英文摘要：

[Objectives] In this study,the effects of Deoxynivalenol（DON） on phenotype and phagocytosis of murine bone marrow-derived dendritic cells induced by lipopolysaccharide（LPS） were investigated.[Methods] The murine bone marrow cells from C57BL/6 mouse were cultured and induced to immature dendritic cells （iDC）.iDCs were exposed to DON（0,125,250 and 500 ng·mL^-1） for 6 h before adding LPS（1 μg·mL^-1）,and then further incubated for 18 h.Cells only incubated with LPS were used as positive controls.The phenotypic changes （CD80,CD86 and MHC-Ⅱ） and phagocytic ability of FITC-dextran in DC were analyzed by Flow cytometry.Relative expressions of cytokines （IL-6,IL-10,IL-12 and TNF-α） were assayed by real-time PCR （RT-PCR）.[Results] The phenotypic expressions of CD80,CD86,and major histocompatibility complex（MHC-Ⅱ）were up-regulated on LPS-stimulated immature dendritic cells（P〈0.05）.However,the phagocytic ability of FITC-dextran was reduced.The gene expressions of cytokines （IL-6,IL-10,IL-12 and TNF-α）were increased （P〈 0.05）.Meanwhile,we found that the phenotypic and immune function of LPS-induced DCs at the level of 125 and 250 ng＆#183; mL-1 DON were not changed （P〉0.05）.The expressions of phenotypic markers（CD80,CD86,MHC-Ⅱ）were down-regulated at the level of 500 ng＆#183; mL-1 DON（P〈0.05）.At the same time,gene expressions of IL-6,IL-10,IL-12,and TNF-α were reduced （P〈0.05）.However,the phagocytic ability of FITC-dextran was elevated by 12.2%.[Conclusions] Our data indicated that DON would play a negative role in the maturation of DCs induced by LPS.This research provided the experimental basis for the immune suppression induced by DON.