中文摘要：

从伏马菌素B1（FB1）杂交瘤细胞株F3中扩增出‰和K基因片段，再经重叠延伸PCR（SOE—PCR）拼接扩增得到单链抗体（ScFv）基因，然后克隆到pCANTAB5E噬菌粒载体上，转化感受态大肠杆菌TGl，并经辅助噬菌体M13K07超感染，构建FB1毒素噬菌体单链抗体库，ScFv基因插入率为100％，库容约为1．5×10^7，噬菌体的滴度为2．1×10^15PFU·mL^-1。免疫亲和筛选和富集后，经ELISA法最终筛选得到5株可分泌阳性噬菌体抗体的细菌。结论：伏马菌素B1噬菌体单链抗体库构建成功。

英文摘要：

The VH and VL gene fragments were first amplified from hybridoma cell line（ F3 ）specific to fumonisin B1 （FBj）integrated as single-chain antibody（ ScFv）gene using gene splicing by overlap extension PCR （SOE-PCR）. The ScFv was cloned into a phagemid vector pCANTAB 5E, and then was transformed into Escherichia coli TG1 to establish a phage ScFv library with the helper phage M13K07. The results indicated that the insert rate of SvFv gene was 100% ,the phage antibody contained 1.5×10^7 independent clones and phage titer was 2.1 × 10^15 PFU· mL^-1. After biopanning enrichment and screening, five strains that could secrete the FB1 specific phage single-chain antibody were selected successfully. Conclusion ：the phage ScFv library against FB~ was constructed successfully.