中文摘要：

目的：观察旋覆代赭汤对反流性食管炎（RE）模型大鼠食管黏膜组织Na＋-K＋-ATP酶及Ca2＋-Mg2＋-ATP酶活性的影响,探讨RE食管黏膜细胞能量代谢障碍与黏膜损伤的关系以及旋覆代赭汤治疗RE的作用机制.方法：将96只雄性Wistar大鼠随机分成6组,即假手术组、模型组、旋覆代赭汤组高、中、低剂量组（24,12,6 g·kg-1）及西药对照组（奥美拉唑10mg·kg-1 ＋2 mg·kg-1莫沙必利）,每组16只.采用“食管-十二指肠端侧吻合术”制备胃及十二指肠液混合RE模型.从术后第3天开始,假手术组、模型组给予生理盐水灌胃,药物治疗组分别给予旋覆代赭汤高、中、低剂量、西药（奥美拉唑10 mg·kg-1＋莫沙必利2 mg·kg-1）灌胃,连续7天,记录大鼠体重变化及死亡情况,于第8天处死大鼠留取标本,观察食管下段黏膜大体及病理组织学变化；化学法检测食管黏膜组织Na＋-K＋-ATP和Ca2＋-Mg2＋-ATP酶活性.结果：与假手术组比,模型组大鼠体重明显减轻（P＜0.05）,食管黏膜肉眼及镜下病理改变明显,评分增高（P＜0.05）；食管黏膜组织Na＋-K＋-ATP酶及Ca2＋-Mg2-ATP酶活性显著降低（P＜0.05）；与模型组及西药组比,旋覆代赭汤高、中剂量组大鼠死亡率明显降低（P＜0.05）：与模型组比,旋覆代赭汤高、中剂量组、西药组肉眼及病理评分显著降低（P＜0.05）；食管黏膜组织Na＋-K＊-ATP酶及Ca2＋-Mg2＋-ATP酶活性明显提高（P＜0.05）.结论：旋覆代赭汤能明显提高RE大鼠食管黏膜细胞膜Na＋-K＋-ATP酶及Ca2＋-Mg2＋-ATP酶活性,从而保持食管黏膜细胞完整性,减轻黏膜损伤.

英文摘要：

Objective：To observe Na＋-K＋-ATPase and Ca2＋-Mg2＋-ATPase activity of esophageal mucosa in reflux esophagitis （RE） rats,and to investigate the fundamental pathogenesis of RE and the therapeutic mechanism in treating RE with Xuanfu Daizhe decoction.Method：Ninty six male Wistar rats were divided into 6 groups：sham operation group,model group,Xuanfu Daizhe decoction high,middle and low dose group （24,12,6 g · kg-1） and western medicine （OME 10 mg · kg-1 plus Mosapride 2 mg · kg-1） group,16 rats each group.Esophagus-duodenum anastomosis was used to establish rat RE model.From 3 days after operation,NS was given to the sham operation group rats and model group rats by gavage feeding; Xuanfu Daizhe decoction and western medicine （OME 10 mg· kg-1 plus mosapride 2 mg · kg-1） was given to the medicine treated groups,and execute all rats in the 8th day,then observe mucous gross change of lower esophagus with eyes and optical microscope.At last,detect the expression of enzymatic activity of Na＋-K ＋-ATP and Ca2＋-Mg2＋-ATP by chemical methods.Result：Compared with sham operation group,the mortality of the model group was higher; the pathological changes and score were increased; the Na＋-K＋-ATPase and Ca2＋-Mg2＋-ATPase of esophageal mucosa were decreased.The mortality of the high and middle dose group of Xuanfu Daizhe decocton treated group was decreased significantly compared with the model group （P ＜ 0.05）.The rats weight of Xuanfu Daizhe decoction treated group were increased significantly compared with the model group （P ＜0.05）.The pathological changes and score were all reduced in Xuanfu Daizhe decoction high and middle dose group （P ＜ 0.01） ; both Na ＋-K ＋-ATPase and Ca2＋-Mg2 ＋-ATPase of esophageal mucosa were increased in Xuanfu Daizhe decoction high and middle dose group （P ＜0.01）.Conclusion：Xuanfu Daizhe decoction could increase the activity of Na＋-K ＋-ATPase and Ca2＋-Mg2＋-ATPase,and protects the cellular integrity of esophageal