中文摘要：

目的：观察旋覆代赭汤及其拆方对反流性食管炎（RE）模型大鼠线粒体腺苷酸转运体（ANT）转运活性的影响。方法：将60只雄性Wistar大鼠,随机分成6组,即正常组、模型组、西药组,旋覆代赭汤全方组及拆方组（包括苦降组、甘升组）,每组10只大鼠。对除正常组外的造模组大鼠采用＂4.2 mm幽门夹＋胃底2/3结扎术＂制备RE动物模型。从术后第7天开始,正常对照组、模型对照组给予生理盐水灌胃,旋覆代赭汤全方组、拆方各组及西药组分别给予相应药液灌胃,干预14 d后,第22天处死全部大鼠后,取材,在光学显微镜下观察RE大鼠病理分级,各组随机取6份食管标本匀浆制成线粒体悬浮,液通过H3-ADP掺入法测量检测食管线粒体ANT转运活性。结果：各组大鼠食管黏膜病理形态学积分比较：模型组大鼠食管黏膜病理积分比正常组显著升高,有显著性差异（P〈0.01）;全方组、西药组、甘升组食管黏膜病理形态学积分比模型组明显降低,差异有统计学意义（P〈0.05）,苦降组食管黏膜病理形态学积分较模型组稍有降低,但差异无统计学意义（P〉0.05）;全方组及西药组食管黏膜病理形态学积分较甘升组稍降低,但差异无统计学意义（P〉0.05）。各组食管线粒体ANT转运活性比较：与正常组比较,模型组、西药组、全方组、甘升组大鼠食管线粒体ANT转运活性略提高,差异无统计学意义（P〉0.05）,苦降组略降低,差异无统计学意义（P〉0.05）;与模型组比较,西药组、全方组及甘升组大鼠食管线粒体ANT转运活性略提高略升高,但差异无统计学意义（P〉0.05）;西药组、全方组、甘升组及苦降组组间比较均无统计学意义（P〉0.05）。结论：旋覆代赭汤通过调节脾胃气机改善RE模型大鼠食管黏膜光镜下表现,促进黏膜修复,改善炎症。在一定程度上提高RE大鼠线粒体ANT转运活性,辅助线粒体能量的?

英文摘要：

Objective： To observe the influence of Xuanfu Daizhe Decoction and its disassembled prescriptions towards the mitochondrial adenine nucleotide translocator（ ANT） activity of the model rats with reflux esophagitis（ RE）. Methods： Sixty male Wistar rats were randomly divided into six groups,namely the normal control group,the model control group,the whole Xuanfu Daizhe Decoction group,the disassembled groups of Xuanfu Daizhe Decoction（ included the Gansheng group,the Kujiang group）as well as the western medicine group（ Lansoprazole ＋ Mosapride）,10 rats in each groups. The model rats were prepared as animal models of RE by adopting 4. 2 mm Helicobacter clip and ligation two- third of the fundus of stomach. After seven days of operation,rats in normal control group and the model control group were lavaged with normal saline while the whole Xuanfu Daizhe Decoction group and its disassembled groups were given the corresponding medicine. Besides,those rats in western medicine group were lavaged with Lansoprazole and Mosapride. After 14 days of intervention,all rats were put to death by day 22,and then observe the morphological changes of the mucosa which was the lower part of the esophageal by optical microscope and observe the activity of esophageal mitochondrial ANT. Results： The integration of the pathological changes： the model group’s was significantly higher than the normal group’s（ P 〈 0. 01）. The whole prescription group,the western medicine control group and the Gansheng group’s were significantly lower than the model group’s（ P 〈 0. 05）. The Kujiang group’s was slightly lower than the model group’s（ P 〉 0. 05）. The whole prescription group and the western medicine control group’s was slightly lower than the Gansheng group’s（ P 〉 0. 05）. The activity of esophageal mitochondrial ANT： the activity of esophageal mitochondrial ANT in the whole prescription group,the model control group,the western medicine group and the Gansheng group’s increased