中文摘要：

背景：研究证明骨髓基质干细胞与煅烧骨支架材料结合后可形成组织工程化骨,但在动物体内的生物相容性及皮下诱导成骨的能力国内报道较少. 目的：观察骨髓基质细胞复合异种煅烧骨植入BALB/c裸鼠背部皮下的成骨性能及煅烧骨材料作为组织工程骨支架材料的可行性. 方法：选用经脱脂及脱蛋白处理后高温煅烧形成的骨支架材料与梯度密度离心法分离培养至第3代的羊骨髓基质干细胞构建细胞-煅烧骨复合物植入 BALB/c 裸鼠背部皮下,选同期对侧背部皮下植入单纯煅烧骨为对照组. 结果与结论：煅烧后的松质骨块为白垩色,表面呈蜂窝状多孔结构,保留了天然松质骨的多孔状空间结构.骨小梁结构完整,孔隙相互连通.骨髓基质干细胞接种到煅烧骨后24 h可见大量细胞黏附于支架上,7 d后细胞分泌大量细胞外基质,细胞与基质分界不清,细胞能在材料上良好地黏附、增殖与生长,细胞活性未受到支架材料的影响.植入4周后,两组均可见煅烧骨边缘出现少量残片,细胞-煅烧骨复合物组煅烧骨孔隙周边可发现骨细胞,对照组煅烧骨表面可见纤维结缔组织包绕.植入后8周,两组均可见到煅烧骨部分降解为片状类骨质,周围有成纤维细胞包绕,排列紧密,形态多样,细胞-煅烧骨复合物组煅烧骨孔隙内可见煅烧骨表面有排列成行的成骨细胞,孔隙间有散在淋巴细胞浸润.对照组标本可见孔隙内有大量结缔组织长入,未见明显成骨迹象.结果说明,经高温煅烧后的松质骨材料,具有良好的生物相容性和生物安全性,可作为骨髓基质干细胞的良好载体,复合后植入体内能够诱导新生骨组织形成,可作为骨缺损组织工程修复的支架材料.

英文摘要：

BACKGROUND：It has proved that the bone marrow stromal cel s and sintered bone support material combination can form tissue-engineered bone, but relevant studies on its biocompatibility in animals and subcutaneous osteogenesis ability are less reported in China. OBJECTIVE：To observe the osteogenesis capacity of marrow stromal stem cel s combined with sintered bone implanted into Balb/c nude mouse back subcutaneously and to explore the feasibility of sintered bone as a scaffold for tissue-engineered bone. METHODS：Sintered bone scaffold materials were prepared using defatted and deproteinized processing and high-temperature calcinations. Then the sintered bone was combined with passage 3 sheep bone marrow stromal stem cel s cultured using density gradient centrifugation method to be implanted subcutaneously into the back of BALB/c nude mice. Simple sintered bone that was subcutaneously implanted into the contralateral back of BALB/c nude mice served as control group. RESULTS AND CONCLUSION：After calcinations, sintered cancel ous bone block colored chalks and the surface displayed a honeycomb porous structure, maintaining the porous structure of the natural cancel ous bone. Bone trabecular structure was of integrity, and pores were mutual y interconnected. After bone marrow stromal stem cel s were inoculated to the calcined bone, there were a lot of cel s adherent to the scaffold within 24 hours, and a large amount of extracel ular matrix at 7 days. The boundary between cel s and extracel ular matrix was unclear, and the cel s could grow and proliferate wel on the scaffold, suggesting that the cel viability was not influenced by the scaffold. Four weeks after implantation, a few of fragments were visible at the edge of sintered bone in the two groups. Bone cel s could be seen around the pores of sintered bone in the combination group, while fibrous connective tissue enveloped the sintered bone in the control group. Eight weeks later, the sintered bone partial y degraded into osteoid sheets surrounded by fibro