采用SBR厌氧氨氧化反应器,研究了不同TOC与NH_4~+-N比值对厌氧氨氧化反应器的脱氮效能的长短期影响.结果表明,在有机物短期影响时,反应器所能承受的最大TOC/NH_4~+-N为1.4,总氮去除速率可达0.26 kg·(m~3·d)~(-1).长期影响下,在TOC/NH_4~+-N小于0.4时,反应器可获得最高脱氮效能,总氮去除率为0.34 kg·(m~3·d)~(-1),TOC/NH_4~+-N大于0.4后,反应器脱氮效能持续降低,并且短期内厌氧氨氧化菌难以迅速恢复活性.利用q PCR(定量PCR)技术对长期影响前后反应器内菌种群落变化做定量分析,结果表明随着有机物的增加,反应器中的ANAMMOX菌数量从2.9×10~(11)copies·mL~(-1)减少至3.15×10~(10)copies·mL~(-1),在TOC/NH_4~+-N大于1.6的环境中,NH_4~+-N未能由厌氧氨氧化菌去除,厌氧氨氧化菌不能表现出生物活性.此时测得反硝化菌数量为3.0×10~9copies·mL~(-1),反应器中的NO_2~--N绝大部分由反硝化去除,虽然反硝化菌数量远少于ANAMMOX菌,但能表现出远超ANAMMOX菌的活性.
The effect of the TOC to NH_4~+-N ratio on nitrogen removal rate in an ANAMMOX sequencing batch reactor was studied for the short term and long term. Short-term results showed the maximum TOC/NH_4~+-N that ANAMMOX could afford was 1. 4. At the same time,the rate of nitrogen removal was 0. 26 kg·( m~3·d)~(-1). The maximum nitrogen removal rate of 0. 34 kg·( m~3·d)~(-1)was obtained when TOC/NH_4~+-N was less than 0. 4 in the long-term experiment. Whereas the nitrogen removal rate of the reactor decreased continuously when TOC/NH_4~+-N exceeded 0. 4,and it was difficult to recover the activity of the ANAMMOX bacteria in the short term.q PCR results showed that the amount of ANAMMOX bacteria dropped from 2. 9 ×10~(11)copies·mL~(-1)to 3. 15 × 10~(10)copies·mL~(-1)with the increase of organics. When TOC/NH_4~+-N exceeded 1. 6,NH_4~+-N could hardly be removed by the ANAMMOX bacteria,which were severely inhibited. The number of denitrifying bacteria was 3. 0 ×10~9copies·mL~(-1); meanwhile,most of the NO_2~--N in the reactor was removed via denitrification.