氨氮是厌氧氨氧菌主要基质之一,但常常因浓度过高而产生脱氮速率不稳定,甚至微生物活性抑制的现象.为了有效避免氨氮对厌氧氨氧化菌活性的抑制,从抑制物形态、主要影响因素和抑制规律探讨了氨氮对厌氧氨氧化菌活性的影响及调控.结果表明,温度、pH值变化对氨氮、游离氨的形态及浓度变化产生重要影响.在恒定进水氨氮浓度500mg/L的情况下,将抑制状态下的pH值从7.9下降到7.3,经过44h运行厌氧氨氧化菌活性获得恢复.在不同进水氨氮浓度下,FA对厌氧氨氧菌活性的半抑制浓度不一样.半抑制浓度与抑制时间存在一定的曲线关系(y=732.38x-0.89).因此,恒定氨氮浓度的条件下,可以通过改变pH值避免FA对厌氧氨氧化菌活性的影响.在不同进水氨氮浓度下,除了考虑降低pH值,还可以通过缩短HRT来避免FA对厌氧氨氧化菌活性的影响.
NH4+-N was one of the important substrate for ANAMMOX bacteria.But instable nitrogen removal processoften occurred and microbial activity even had inhibited by using ANAMMOX bacteria,because of high substrateconcentration.In order to effectively avoid NH4+-N inhibition to anammox bacteria activity,the effect of NH4+-N on theactivity of anammox bacteria was analysis by inhibitor morphology,main influence factors and inhibiting regularity.Theresults showed that temperature and pH were important impact on morphology and concentration changes betweenNH4+-N and FA.The ANAMMOX activity was recovered after44h operation when the pH was decreased from7.9to7.3under the influent concentration of NH4+-N was fixed at500mg/L.Half maximal inhibitory concentration(IC50)of FAon ANAMMOX was different when the influent concentration of NH4+-N was variety.IC50of FA and inhibitory timeunder different NH4+-N concentrations have relationship(y=732.38x-0.89).Thus,we avoided the effect of FA on activity ofanammox bacteria by changing the pH when the influent concentration of NH4+-N was constant.Besides pH decreased,HRT can also be shortened to avoid the effect of FA on activity of ANAMMOX bacteria,when the influent concentrationof NH4+-N was fluctuate.