中文摘要：

目的探讨人食管鳞状细胞癌中缺氧诱导因子-1α（HIF-1α）和已糖激酶-Ⅱ（HK-Ⅱ）的表达变化及对糖酵解的影响。方法缺氧条件（1％氧浓度）下培养TE13及Eca109细胞，设置不同缺氧时间（分别为6、12、24、48h），并以常氧培养（20％氧浓度）为对照。Western印迹检测HIF-1α及HK-Ⅱ的蛋白表达变化；经RNA干扰技术特异性静默HIF-1α，利用实时定量PCR及Western印迹检测HIF-1α和HK-Ⅱ的表达变化；分光光度法检测常氧和缺氧条件下细胞培养液中细胞的乳酸浓度变化。结果①缺氧条件下，TE13及Eca109细胞中HIF-1α表达均随缺氧时间延长而逐渐升高（P〈O．05），在缺氧12h表达达到高峰，后表达又随时间延长而下降。TE13及Eca109细胞缺氧12h后HK-Ⅱ表达均较常氧培养明显增强（P〈0．05）。②实时定量PCR法检测显示，常氧条件下被干扰的TE13／shRNA和Eca109／shRNA细胞中HIF-1dRNA表达量较未干扰的TE13、Eca109细胞明显减弱（P〈O．05）。HK-ⅡRNA表达结果与HIF-1 RNA一致。③常氧及缺氧培养时，HK-Ⅱ蛋白在TE13／shRNA和Eca109／shRNA细胞的表达均较未干扰的TE13、Eca109细胞明显减弱，差异有统计学意义（P〈0．05）。④缺氧时TE13和Eca109细胞乳酸分泌量为14．707±3．594和15．062±3．901，较常氧时增多（6．070±1．839和6．891±1．592，P〈0．05）。TE13／shRNA、Eca109／shRNA乳酸分泌量较未静默TE13、Eca109细胞显著减少，差异均有统计学意义（P〈0．05）。结论食管鳞癌中HIF-1α及HK—Ⅱ在缺氧条件下表达明显增强，HK—Ⅱ表达及乳酸浓度与HIF-ICt表达密切相关，HIF-1α可能通过HK-Ⅱ影响细胞的糖酵解水平。

英文摘要：

Objective To investigate the changes of hypoxia-inducible factor （HIF）-1α and hexokinase-Ⅱ （HK-Ⅱ） expression in human esophageal squamous cell carcinoma and its effect in glycolysis. Methods TEl3 cells and Ecal09 cells were cultured under hypoxic condition （1 % O2） for different hypoxic time （6, 12, 24 and 48 hours）. Cells cultured under normal oxygen condition （20% O2 ） were set as control. The changes of HIF-1α and HK-Ⅱ expressions at protein level were detected by Western blot. HIF-1α genes were specifically silenced with RNA interference technology （RNAi）, and then the changes of HIF-1α and HK-Ⅱ expression were determined by realtime PCR and Western blot. Under normal oxygen and hypoxic condition, the changes of lactic acid concentration in cell culture medium were detected by spectrophotometric method. Results Under hypoxic condition, the expression of HIF-1α and HK-Ⅱ gradually increased as hypoxic time extended （P〈0.05）, reached a peak at 12h and then gradually decreased as time extended. Compared with that under normal oxygen condition, the expression of HK-Ⅱ in TE13 cells and Eca109 cells significantly increased under hypoxic condition （P〈0.05）, which was more significant after 12 hours hypoxia. The result of realtime PCR indicated that under normal oxygen condition the expression of HIF-1α at RNA level in TE13/shRNA cells and Eca109/shRNA cells significantly decreased compared with TEl3 cells and Eeal09 cells without interference （P%0. 05）. The expression of HK-Ⅱ at RNA level was consistent with the result of HIF-1α. Under normal and hypoxia condition, the expression of HK-Ⅱ at protein level in TE13/shRNA cells and Ecal09/shRNA cells significantly decreased compared with TE13 cells and Ecal09 cells without interference, and the difference was statistic significant （P〈0.05）. The lactic acid secretion of TE13 cells and Ecal09 cells under hypoxia condition （14. 707±3. 594 and 15. 062±3. 901） was higher than that under normal oxygen c