中文摘要：

啤酒中含有大量胺类物质，其种类和含量与啤酒口感与质量密切相关。本文以异硫氰酸荧光素（FITC）为衍生试剂，高效液相色谱（HPLC）-激光诱导荧光（LIFD）检测，分析啤酒中含有-NH2和-NH-基团的胺类物质；低温真空蒸馏结合气相色谱-氢火焰离子化（GC-FID）测定啤酒中亚硝胺类物质含量，建立了啤酒中胺类物质的系统分析方法。进-步以该方法，对市售多种啤酒进行了系统分析。结果表明，不同啤酒中胺类物质的种类及其含量均具有-定差异。以峰高值lmV为积分阈值，样品色谱图中最多检测出53个色谱峰，最少检测出38个色谱峰；对三种啤酒样品中亚硝胺分析，在两种啤酒样品中检测到微量亚硝胺。该方法灵敏度高，稳定性好，操作简单，可用于啤酒质量及伪劣产品检测，还可以用于其它饮料及食品中胺类物质分析。

英文摘要：

Many amine compounds are exist in Beer. Whose taste and quality are closely related to the content and the kind of the amine compounds. A method for trace amine compounds analysis in beer was developed in this paper. The amine compounds withNH2 and NH in beer were analyzed by HPLC separation and LIFD determination, using FITC as derivative regent. Then the nitrosamines in beer were quantitative analyzed by lowtemperature vacuum distillation com bined with GCFID. Further more, various kinds of beer on sell were analyzed. Using the developed method, the result indicated that there existed some differences of amine compounds among different brands and different series of beer. Setting the integral threshold at lmv, the largest number of HPLC peaks is 53 and the least number is 38. In the chromato grams of beer samples, Analyze the content of nitrosamines in three kinds of beer and trace nitrosamines have been de tected in two kinds of them. The method has a high sensitivity, good stability and is easily operated. It can be used for de tection of quality of beer and fake and shoddy products and is valuable significance to control the content of carcinogenic amine compounds. It is very important for the health of people. The method is also suitable for amine compounds analysis in other beverage and food.