欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
蛋白质组学是后基因时代生命科学研究的核心内容之一,其中发展高分辨、高灵敏度、高准确性和高通量的分析技术至关重要。整体材料具有制备简单、传质速度快、色谱柱反压低、表面易修饰等优点,因此已被广泛用于蛋白质组学研究中。本文对各种整体材料（包括有机聚合物整体材料、硅胶整体材料、有机-无机杂化整体材料）的制备方法及其在蛋白质组学研究（如蛋白质的酶解、蛋白质和肽段的分离以及在线酶解-分离-鉴定的高通量分析）中的应用进行了系统综述。
英文摘要：
Proteomics is one of the core contents of life science in the post-genomic era,among which it is very important to develop the analytical techniques with high resolution,high sensitivity,high accuracy and high throughput.With the advantages of facile preparation,fast mass transfer,low backpressure and easy modification,monolithic materials have been widely used in proteomic analysis.This review summarizes the preparation methods of different kinds of monolithic materials（including organic polymer monoliths,silica-based monoliths,organic-inorganic hybrid silica monoliths） and their applications in proteomic study such as the digestion of proteins,the separation of proteins or peptides,high throughput analysis integrating online digestion,separation and identification.
同期刊论文项目
同项目期刊论文
Development of a Highly Efficient 2-D System with a Serially Coupled Long Column and Its Application
High throughput tryptic digestion via poly (acrylamide-co- methylenebisacrylamide) monolith based im
Octyl-functionalized hybrid magnetic mesoporous microspheres for enrichment of low-concentration pep
Coupling formic acid assisted solubilization and online immobilized pepsin digestion with strong cat
Facile preparation of monolithic immobilized metal affinity chromatography capillary columns for sel
Integration of capillary isoelectric focusing with monolithic immobilized pH gradient, immobilized t
Piperazines for peptide carboxyl group derivatization: effect of derivatization reagents and propert
Nano-flow multidimensional liquid chromatography platform integrated with combination of protein and
On-line combination of monolithic immobilized pH gradient-based capillary isoelectric focusing and c
Smad4 mediates malignant behaviors of human ovarian carcinoma cell through the effect on expressions
Macroporous molecularly imprinted monolithic polymer columns for protein recognition by liquid chrom
Recent advances in micro-scale and nano-scale high-performance liquid-phase chromatography for prote
Highly efficient proteome analysis with combination of protein pre-fractionation by preparative micr
A hydrophilic immobilized trypsin reactor with N-vinyl-2-pyrrolidinone modified polymer microparticl
Hydrophilic monolith based immobilized enzyme reactors in capillary and on microchip for high-throug
Analysis of low molecular weight acids by monolithic immobilized pH gradient-based capillary isoelec
Organic-Inorganic Hybrid Silica Monolith Based Immobilized Titanium Ion Affinity Chromatography Colu
NSI and NSMT: usages of MS/MS fragment ion intensity for sensitive differential proteome detection a
期刊信息
位置：