中文摘要：

三叉神经痛（trigeminalneuralgia，TN）是局限于三叉神经1支或多支分布区域的反复发作的短暂剧烈疼痛，临床发病率高，严重影响患者生活质量，难以完全治愈。本研究旨在探讨川芎嗪（tetramethylpyrazine，TMP）对慢性压迫性损伤眶下神经（chronicconstrictioninjuryoftheinfraorbitalnerve，ION-CCI）致大鼠TN的影响。Sprague—Dawley大鼠随机分为4组：假手术组（sham）、假手术＋川芎嗪组（sham＋TMP）、模型组（TN）、模型＋川芎嗪组（TN＋TMP）。通过ION—CCI建立大鼠TN模型，术后2周每天一次腹腔注射川芎嗪（50mg／kg）。应用电子测痛仪观察各组大鼠机械痛敏阈值的变化。同时采用RT-PCR、蛋白印迹、免疫组织化学染色等方法检测大鼠术侧三叉神经节（trigeminalganglia，TGs）中降钙素基因相关肽（calcitoningene-relatedpeptide，CGRP）的表达变化。术后15天内，TN组大鼠手术侧眶下神经面部感觉区域的机械痛敏阈值和sham组相比明显降低，术后第9天到第15天，TN＋TMP组和TN组相比较阈值出现明显升高沪〈0．05）。术后第15天，RT-PCR、蛋白印迹、免疫组织化学染色结果表明，与sham组相比，TN组大鼠TGs中CGRP表达明显升高（P〈0．05）；与TN组相比，TN＋TMP组大鼠TGs中CGRP表达显著降低（P〈0．05）。以上结果表明，TGs中的CGRP对TN的痛觉传递有着重要作用，TMP对TN有一定的抑制作用，其机制可能和抑制TGs中CGRP表达有关。

英文摘要：

Trigeminal neuralgia （TN） is a kind of recurrent transient and severe pain that is limited to the trigeminal nerve in one or more branches. The clinical incidence of TN is high, which seriously affects the quality of life of the patients and is difficult to cure. The present study investigated the effects of tetramethylpyrazine （TMP） on TN induced by chronic constriction injury of the infraorbital nerve （ION-CCI） in rats. Adult male Sprague-Dawley rats were randomly assigned to four groups： sham, sham treated with TMP （Sham＋TMP）, TN model （TN）, and TN treated with TMP （TN＋TMP）. The rat TN model was established by ION-CCI and TMP （50 mg/kg） was injected intraperitoneally once a day for 2 weeks after operation. The mechanical response threshold was tested by theelectronic von Frey filaments. The expression of CGRP in the trigeminal ganglia （TGs） of rats on the operative side was detected by RT-PCR, immunohistochemical staining and Western blot. In 15 days after operation, TN group showed a robust decrease in mechanical response threshold as compared with sham group. From day 9 to day 15 after operation, TMP treatment significantly suppressed the TN-induced mechanical hyperalgesia （P 〈 0.05）. On day 15 after operation, RT-PCR, immunohistochemical staining and Western blot analysis showed an obvious increase in expression level of CGRP in TGs of TN group compared with sham group, which was downregulated by TMP treatment （P 〈 0.05）. These results suggested that TMP might have a therapeutic potential for the treatment of TN through regulating CGRP expression in the TGs.