中文摘要：

目的探究大黄素（emodin）对P2X2/3受体介导的三叉神经痛的作用影响。方法慢性压迫性损伤大鼠眶下神经（CCI-ION）建立大鼠三叉神经痛动物模型,应用von Frey细丝法测试机械痛敏阈值,观察大黄素对三叉神经痛（TN）大鼠机械痛敏阈值的影响。结合原位杂交、PT-PCR、免疫组织化学、免疫荧光、蛋白印迹等方法观察大鼠三叉神经节（TG）中P2X2/3受体的表达变化。结果术后14 d,Ⅲ组（TN模型组）和Ⅰ组（生理盐水对照组）、Ⅱ组（假手术组）、Ⅳ组（TN模型＋大黄素干预组）相比较,大鼠手术侧眶下神经面部感觉区域机械痛敏阈值明显降低（P〈0.01）,大鼠TG中P2X2/3受体表达明显升高（P〈0.01）;Ⅰ、Ⅱ、Ⅳ组之间相比,大鼠手术侧眶下神经面部感觉区域机械痛敏阈值差异没有显著性（P〉0.05）;Ⅳ组TG中P2X2/3受体的表达较Ⅲ组低（P〈0.01）。结论 P2X2/3受体涉及到三叉神经痛,大黄素对P2X2/3受体介导的三叉神经痛有一定抑制作用。

英文摘要：

Aim To explore the role of emodin in trigeminal neuralgia mediated by P2X2/3 receptor.Methods The trigeminal neuralgia rat model（a chronic constriction injury of the infraorbital branch of the trigeminal nerve,ION-CCI） was established.The mechanical hyperalgesia threshold of trigeminal neuralgia（TN） rats was tested by the von Frey filaments.The change of the expression of P2X2/3 receptors in rat trigeminal ganglion（TG） was detected with in situ hybridization,RT-PCR,immunohistochemical staining,immunofluorescence,and Western Blot.Results 14 days after operation,compared withⅠ group（saline control group）,Ⅱ group（sham operation group） or Ⅳ group（TN model＋emodin intervention group）,Ⅲ group（TN model group） had a significant decrease of mechanical hyperalgesia threshold in the territory of ligated infraorbital nerve（P0.01）,and an obvious increase in expression of P2X2/3 receptor in rat TG（P0.01）;there was no significant differences of mechanical hyperalgesia threshold amongⅠ,Ⅱ and Ⅳ group（P0.05）;the expression of P2X2/3 receptor of TG in Ⅳ group was lower than that in Ⅲ group（P0.01）.Conclusion P2X2/3 receptor may play a key role in the pain transmitting of trigeminal neuralgia,and emodin has inhibitory effect on trigeminal neuralgia.