欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
为提高重组毕赤酵母生产碱性果胶酶的产量和生产强度，在摇瓶条件下优化了重组毕赤酵母生产碱性果胶酶的关键因素。结果表明，以下条件：初始甘油浓度40g／L、初始甲醇浓度3.1g甲醇／gDCW、每24h添加0.51g甲醇／gDCW、诱导表达周期72h、250mL三角瓶诱导培养基装液量30mL、初始pH6,0，最适于菌体生长与产物表达。在此基础上，7L罐上通过恒速流加甘油进一步提高细胞密度，诱导阶段甲醇采取前期恒速流加和后期DO-stat，发酵结束菌体干重达80g／L，酶活为217U／mL，比摇瓶结果提高了66.2％。
英文摘要：
The key factors on high-level polygalacturonate lyase （PGL） production in recombinant Pichia pastoris were investigated. In 250 mL shake flask, the optimal glycerol concentration, initial methanol concentration, methanol supplementation quantity, duration of induction, initial pH, medium volume were 40 g/L, 3.1 g Methanol/g DCW, 0.51 g Methanol/g DCW （in every 24 h）, 72 h, pH6.0 and 30 mL, respec tively. In 7 L fermentor, constant glycerol feeding, constant and DO-stat methanol feeding strategies were applied to enhance cell density and PGL production. Finally, the dry cell weight was 80 g/L, and the maximum yield of PGL was achieved to 217 U/mL, increased by 66.2% than that of flask level.
同期刊论文项目
同项目期刊论文
Delayed supplementation of glycine enhances extracellular secretion of the recombinant alpha-cyclode
Application of a novel cavern volume controlled culture model to microbial hyaluronic acid productio
Characterization of Thermobifida fusca Cutinase-Carbohydrate-Binding Module Fusion Proteins and Thei
Improved production of polygalacturonate lyase by combining a pH and online methanol control strateg
Heterologous Expression and Biochemical Characterization of alpha-Glucosidase from Aspergillus niger
Modeling and optimization of cutinase production by recombinant Escherichia coli based on statistica
Enhanced production of poly(vinyl alcohol)-degrading enzymes by mixed microbial culture using 1,4-bu
Combined dissolved oxygen and pH control strategy to improve the fermentative production of l-isoleu
Typical methanogenic inhibitors can considerably alter bacterial populations and affect the interact
Enhancement of cell viability and alkaline polygalacturonate lyase production by sorbitol co-feeding
Use of Escherichia coli add/ade mutant and Saccharomyces cerevisiae WSH2 to construct a highly effic
Enhanced secretion of recombinant alpha-cyclodextrin glucosyltransferase from E. coli by medium addi
Enhancement of pyruvate productivity by inducible expression of a F0F1-ATPase inhibitor INH1 in Toru
Modeling and Optimization of Microbial Hyaluronic Acid Production by Streptococcus zooepidemicus Usi
Enhancement of glutathione production in a coupled system of adenosine deaminase-deficient recombina
Enhancement of Hyaluronic Acid Production by Batch Culture of Streptococcus zooepidemicus via the ad
Comparative study on the influence of dissolved oxygen control approaches on the microbial hyaluroni
Calcium Leads to Further Increase in Glycine-Enhanced Extracellular Secretion of Recombinant alpha-C
Mutations at subsite-3 in cyclodextrin glycosyltransferase from Paenibacillus macerans enhancing alp
Lowering induction temperature for enhanced production of polygalacturonate lyase in recombinant Pic
Enhanced Hyaluronic Acid Production of Streptococcus zooepidemicus by Shifting Dissolved Oxygen Leve
Microbial production of low molecular weight hyaluronic acid by adding hydrogen peroxide and ascorba
A newly isolated Paecilomyces sp WSH-L07 for laccase production: isolation, identification, and prod
Culture conditions optimization of hyaluronic acid production by Streptococcus zooepidemicus based o
Enhanced cutinase production of Thermobifida fusca by a two-stage batch and fed-batch cultivation st
Enhanced hyaluronic acid production of Streptococcus zooepidemicus by an intermittent alkaline-stres
Influence of hyaluronidase addition on the production of hyaluronic acid by batch culture of Strepto
期刊信息
位置：