中文摘要：

目的：建立无紫外吸收的藏药镰形棘豆总生物碱的定量分析方法。方法：采用酸性染料比色法,以野决明碱为对照品,溴麝香草酚蓝为酸性染料,药材先用酸水提取,碱化后经有机溶剂振摇提取,检测波长为413nm。结果：野决明碱进样量在20.06～100.30μg范围内与吸光度呈良好线性关系（r=0.9995）;平均加样回收率为100.7%,RSD=1.41%（n=6）。结论：该方法简便、准确、可靠,可用作镰形棘豆中总生物碱的定量分析方法,为其质量评价提供科学依据。

英文摘要：

OBJECTIVE： To establish the quantitative method for analysis of total alkaloids of Tibetan medicine Oxytropis falca- ta. METHODS： Samples were extracted with acid water and then shook with organic solvent after alkalization, and the content of total alkaloids in samples was assayed by acidic dye colorimetry using thermopsine as control and bromothymol blue as the acidic dye. The detection wavelength was 413 nm. RESULTS： The linear ranges of thermopsine were 20.06-100.30 pg（r=0.999 5） with an average recovery of 100.7% （RSD=- 1.41%, n= 6）. CONCLUSION： The method is simple, accurate and reliable, and it can be used for quantitative analysis of total alkaloids in O. falcata and provides scientific basis for quality evaluation of it.