为验证在室内采用禾谷丝核菌菌丝悬浮液直接接种小麦发芽种子来评价小麦苗期纹枯病抗性方法的准确性,本研究采用16份小麦品种(系)进行室内幼苗纹枯病抗性、田间苗期纹枯病抗性、温室苗期纹枯病抗性和田间成株期抗性鉴定及相关性分析。结果表明,室内幼苗纹枯病抗性鉴定结果与田间苗期纹枯病抗性鉴定结果显著相关,该方法可用于小麦苗期纹枯病抗性评价,小麦苗期与成株期纹枯病的抗性机制可能不同。采用该鉴定方法对黄淮麦区302份小麦品种(系)进行幼苗纹枯病抗性评价,筛选到5份抗病新种质。这些研究结果将为小麦纹枯病抗病育种工作的开展提供帮助。
In order to validate the accuracy of new evaluation method for wheat sharp eyespot( WSE) resistance of seedling by inoculating directly with the suspension of disease mycelia,sixteen varieties were respectively identified the resistance to WSE by the evaluation methods of seedling indoor,seedling in field,seedling in greenhouse and adult plant in field. The correlation between the evaluation methods was analyzed in this study. The results showed that there was significant correlation between the resistance of seedling indoor and that of seedling in field. The evaluation method of seedling indoor could be used to identify the resistance to WSE in seedling stage. The results also indicated that there could be different resistance mechanisms between seedling and adult plant of wheat. Using the new method,five wheat varieties with the seedling resistance to WSE were screened from 302 varieties of Huanghuai wheat region. These results will be helpful to the breeding of wheat cultivar with the resistance to WSE in the future.