本试验旨在研究维生素A、D对肉鸡钙、磷代谢的影响。采用4×4完全随机试验设计,将384只8日龄健康艾维茵雄性肉鸡称重后随机分为16个处理组,每个处理组设3个重复,每个重复8只。其中,VA设4个添加水平,分别为1500、3000、15000和45000IU/kg日粮;VD设4个添加水平,分别为500、1250、2500和5000IU/kg日粮,共构成16个处理组的试验日粮。结果表明:日粮VA水平对肉鸡钙、磷代谢有显著的影响,日粮VA为45000IU/kg时,肉鸡胫骨灰分含量、胫骨矿化度及血清AKP活性相对于1500、3000IU/kgVA组显著下降(P〈O.01或P〈O.05),胫骨磷含量显著增加(P〈0.05),胫骨钙含量趋于增加(P〉0.05);日粮VA水平为15000IU/kgtt,],胫骨钙含量、血清AKP活性有降低的趋势,胫骨磷含量趋升高。日粮VD水平在500~5000IU/kg范围内,对肉鸡的胫骨灰分含量、胫骨钙、磷含量、胫骨矿化度及血清AKP活性无显著影响(P〉0.05),但随着VD添加剂量的增加,血清钙、磷含量呈一次线性增加,胫骨钙含量呈二次曲线上升趋势。日粮VA为15000IU/kg时,肉鸡VA临界过量;日粮VA为45000IU/kg时,肉鸡VA过量。日粮VD水平在500~5000IU/kg范围内,对肉鸡的钙、磷代谢无显著影响。
The experiment was conducted to elucidate the effects of interaction between vitamin A and vitamin D on metabolism of calcium and phosphorus in broilers. Three hundred and eighty four 8-day old AA male broilers with similar body weight were randomly allotted into 16 treatments with 3 replicates of 8 broilers each. Broilers were fed four levels of dietary vitamin A (1 500,3 000,15 000,and 45 000 IU/kg) and four levels of vitamin D (500,1 000,2 500,and 5 000 IU/kg) in a 4×4×3 factorial arrangement. The results indicated that the bone ash, bone mineral density (BMD) and Alkaline Phospha- tase (AKP) activity of serum was decreased significantly fed on 45 000 IU/kg vitamin A (P(0.01 or P(0.05)and the bone phosphorus was increased significantly (P〈0.05) compared to the addition of 1 500 and 3 000 vitamin A in diets. Bone calcium had the tendency to be increased(P〈0.05)while dietary vitamin A levels was 45 000 IU/kg. While dietary vitamin A levels was 15 000 IU/kg,the bone calcium and serum AKP activity had the tendency to be decreased, and the bone phosphorus was increased. The results also suggested that serum calcium and phosphorus contents increased linearly along with the incre- ment of vitamin D additive dose,and bone calcium increased quadratically while dietary vitamin D level was between 500 and 5 000 IU/kg. The addition of 15 000 IU/kg VA would be referred to as marginally excess for broilers, and the addition of 45 000 IU/kg VA would be excessive for broilers. A dosage level of 50045 000 IU/kg vitamin D had no significant effects on the metabolisms of calcium and phosphorous in broilers.