中文摘要：

目的研究HLA-DRB1等位基因与HBV感染慢性化的相关性。方法用PCR-SSP方法对陕西地区汉族慢性乙肝患者108例与正常对照108人以及慢性乙型肝炎表面抗原携带者32人进行HLA—DRB1等位基因分型比较，并进行HLA-DRB1等位基因分型与HBV复制状态相关性分析。结果陕西地区汉族人HLA—DRB1等位基因以DR-B1＊04（16．2％），DRB1＊09（12．5％），DRB1＊12（11．6％），DR-B1＊15（13．4％）最为常见；病例组HLA—DRB1＊03的等位基因频率（10．6％）明显高于健康对照组（3．7％），（OR=3．10；P〈0．05）；HLA—DKB1＊07等位基因频率病例组（17．6％）明显高于健康对照组（9．3%，（OR=2．09；P〈0．05）；DRB1＊07基因位点在HBV高复制状态多见（OR=2．22；P〈0．05）。结论HLA—DRB1＊03、HLA—DRB1＊07与陕西地区汉族人HBV感染后的慢性化相关联。该研究提示HLA-Ⅱ类基因与HBV感染慢性化有关联。

英文摘要：

[Objective] To study the HLA-DRB1 genotype and their relation with HBV infection among Shaan＇xi Han patients. [Methods] HLA-DRB1 genotyping was conducted in 108 case of chronic HBV infection and 108 health controls as well as 32 asymtomatic HBsAg cartier by using polymerase chain reaction-sequence specific primer method. All the patients, asymtomatic HBsAg cartier and health subjects were residents of Shaan＇xi district and belonged to Han nationality. We also studied the association between HLA-DRB1 genotype and different replication of HBV. [Results] DRB1＊04, DRB1＊09, DRB1＊12, DRB1＊15 were the most common genotypes in the Shaan＇xi Han residents with the frequency of 16.2%, 12.5%, 11.6% and 13.4% respectively. Compared to 108 health controls, the allele frequency of HLA-DRB1＊03 （10.6% of HBV patients versus 3.7% of health controls, odds ratio=3.10; P 〈0.05） and HLA-DRBi＊07 （17.6% of HBV patients versus 9.3% of health controls, odd ratio=2.09; P 〈0.05） were markedly higher. [Conclusion] HLA-DRB1＊03 and HLA-DRB1＊07 are closely related with susceptibility to chronic hepatitis B, and DRB1＊15 is closely related with resistance to chronic hepatitis B. These finding suggest that host HLA class Ⅱ gene is an important factor determining the outcome of HBV infection.