中文摘要：

目的 提供血清中的HBV病毒能进入体外培养的滋养层细胞（HPT-8）的依据。方法 用HBV阳性血清体外感染HPT-848h,设感染组和对照组。ELSIA检测细胞培养上清中HBsAg、HBeAg,PCR检测细胞培养上清和细胞中HBVDNA,细胞免疫组织化学法检测细胞中HBsAg、HBcAg,免疫荧光定量PCR检测细胞培养上清中HBVDNA的含量。结果ELISA检测PBS洗8次后第1、2代感染细胞的培养上清的HBsAg、HBeAg均呈阳性;PCR在感染细胞第1、2代的培养上清中检测到HBVDNA特异的片断;第1、2代感染细胞中检测出了rcDNA,第1代感染细胞中检测出了cccDNA;免疫组化检测感染组第2代细胞中HBsAg阳性;荧光定量PCR检测感染细胞第1、2代培养上清中HBVDNA的滴度分别为8×10^4拷贝/ml和4.6×10^3拷贝/ml,第3代〈10^3拷贝/ml。结论 血清中的HBV病毒能进入HPT-8,但在HPT-8内很难复制、繁殖。

英文摘要：

Objective To provide the evidence of HBV positive serum infecting HPT - 8 cell line. Methods HPT - 8 cells were cultured with HBV positive serum in infection group and with HBV negative serum in control group, 48 h later the inoculums were removed and the cells were extensively washed with PBS. HBsAg and HBeAg in cell culture medium were detected by ELISA method, and PCR method to detected HBV DNA in cell culture medium and cells. Immunohistochemical staining method was used to detect HBsAg and HBcAg in cells. HBV fluorescence polymerase chain reaction diagnose kit were used to detect the HBV DNA concentrations in cell culture medium. Results After PBS washed, HBsAg and HBeAg in infection group cell culture medium at passage 1 and 2 could be detected by ELISA method. HBV DNA was detected by PCR in infection group culture medium at passage 1 and 2. HBV rcDNA was detected by PCR in infection cells at passage 1 and 2. HBV cccDNA was detected by PCR in infection cells at passage 1. Immunocytochemistry method detected HBsAg in infected cells at passage 2. Florescence quantify PCR detected HBV DNA in infect cell culture medium in passage 1, 2, and 3 which titer of 8 × 10^4 , 4.6 × 10^3, 〈 10^3 （copy/m/） respectively. Conclusion HBV positive serum could infect placental trophoblastic cells in vitro.