以商陆为实验材料,用水培方法设置了8个实验处理:Fe0Mn0(对照),FeoMn-,FeoMn+,Fe- Mno,Fe- Mn-.Fe—Mn+,Fe+Mn0,Fe+Mn-,Fe+Mn+。处理30d时。对根系分泌物中可溶性糖、总氨基酸、pH值以及根系质膜透性进行测定。结果表明,Fe—Mn-的可溶性糖含量稍高于对照,其余各处理组均低于对照,Fe0Mn+,Fe+Mn+达到显著水平;氨基酸含量在铁锰失衡条件下普遍有所增加。其中Fe-Mn0分泌的氨基酸含量最大,与对照差异显著;除FeoMn+外,各处理组的pH值与对照组相比都显著增加,其中以铁毒处理组(Fe+Mn0,Fe+Mn+,Fe+Mn-)的增加率最高;铁毒处理组(Fe+Mn0。Fe+Mn+,Fe+Mn-)使商陆根系质膜透性增加,对商陆根系造成一定的伤害。总体而言,铁锰的缺乏或过量都会引起商陆根系分泌物含量的变化,其中以铁胁迫和锰毒对其影响最大;另外,相对单铁或单锰处理而言,铁锰之间的交互作用对商陆根系分泌物的变化也有一定的影响。
The effect of unbanlance between iron and manganese on Phytolacca acinosa Roxb. root exudates ,were investigated with hydroponics under eight experimental group (Fe0Mn-, Fe0Mn+, Fe- Mn0, Fe- Mn, Fe-Mn+, Fe+ Mno, Fe+ Mn-, Fe+ Mn+ ) and one control group(Feo Mn0 ). The soluble sugar, amino acid, pH value and membrane permeability of root exudates were determinated. The results were showed as follows: The soluble sugar content of Fe- Mn- was a little more than control, but the others were less than control, and thoese of Fe0Mn+ and Fe+ Mn+ reached significant level. The amino acid content of most of treatments was higher than control, Fe- Mn0 was most and showed significant difference to control. The pH values of all of treatments significantly increased except Fe0Mn+, the increasing rate of Fe+ Mn0 ,Fe+ Mn+, Fe+ Mn- were biggest, what's more, they made the membrane permeability of root increase. In all, both deficiency and overdose of iron and manganese resulted in changing of root exudates, and the effection of iron stress and manganese toxicity was bigger. In addition, compared to individual iron or manganese treatment, root exudates were also affeced by interaction of iron and manganese.