中文摘要：

[目的]研究大豆根缘细胞发育的生物学特性和对Al^3＋毒害的响应,为提高大豆的耐铝性提供理论依据.[方法]以浙春3号和辽鲜3号为材料,采用悬空气培法,研究大豆根缘细胞产生的数目、活性随根长的变化及Al^3＋毒胁迫下根缘细胞形成和释放的难易程度.[结果]大豆根冠周围新形成的根缘细胞呈圆形,随着生长逐渐发育成椭圆形或矩形或长条形.当根长为15～25 mm时,根缘细胞数目达最大值,浙春3号约3 000个,辽鲜3号约3 800个.去根缘细胞的大豆分别用100、200、300、400 μmol·L^-1 Al^3＋溶液喷洒根尖,根缘细胞的数目和活性明显低于0 μmol·L^-1Al^3＋溶液喷洒的处理,且Al^3＋浓度越高,根缘细胞数目和活性下降幅度越大.200 μmol·L^-1 Al^3＋、300 μmol·L^-1 Al^3＋处理4 h,两个大豆品种的PME活性明显升高,400 μmol·L^-1Al^3＋处理后PME活性均有明显下降.这说明高浓度Al^3＋不利于根缘细胞的形成和释放,降低细胞活性和PME活性,辽鲜3号的根缘细胞对Al^3＋胁迫反应更为敏感.[结论]Al^3＋毒害时,维持较高根缘细胞的数目、活性及PME活性是抗性品种耐铝毒的重要途径.

英文摘要：

[Objective] The biotic characteristics and response of root border cells to Al^3＋ toxicity in soybean can account for. The AI resistance mechanism at the level of border cells. [Method] Experiments were performed with two soybean cultivars （Zhuchun No.3 and Liaoxian No.3） under aeroponic culture. [Result] Border cell shape of both soybean cultivars was spherical and small at the initial stage. As cells developed, they became ellipsoidal or spectacular and long in shape. The number of border cells reached the maximum value （3 000 for Zhuchun No.3 and 3800 for Lianxian No.3） when root length was 15-25 mm. The number and viability of border cells released from roots with 100, 200, 300, 400 μmol·L^-1 Al^3＋ treatment were less than that from roots without Al^3＋ treatment, the production and viability was seriously inhibited with high Al^3＋ concentration. The PME activity increased with 200 μmol·L^-1 Al^3＋ and 300 μmol·L^-1 Al^3＋ for 4 h, whereas remarkably decreased with 400 μmol·L^-1 Al^3＋ treatment. These results suggested that high Al^3＋ concentration inhibited the production and release of soybean and decreased PME activity. Response of border cells to Al^3＋ in Liaoxian No.3 was more sensible than in Zhechun No.3. [ Conclusion ]The number and cell viability and PME activity of border cells were higher in Zhechun No.3 than that in Liaoxian No.3, the different Al^3＋ toxicity response accounted for A1 resistance mechanism in Zhechun No.3.