中文摘要：

小肠结肠炎耶尔森菌作为一种食源性致病菌逐渐引起了关注。本实验利用胰蛋白胨培养基、改良磷酸盐缓冲液和氯苯酚-替卡西林-氯酸钾培养基对小肠结肠炎耶尔森菌前增菌,并采用实时荧光PCR方法对增菌效果进行检验。结果表明：在纯菌体系中胰蛋白胨培养基对小肠结肠炎耶尔森菌的增菌作用优于其他两种培养基,并且增菌24h后即可检测到该菌。在混菌体系中小肠结肠炎耶尔森菌经过改良磷酸盐缓冲液增菌后灵敏性最高,并且增菌时间可缩短至8h。因此在实际检测中可采用改良磷酸盐缓冲液对该菌进行增菌检测。

英文摘要：

Yersinia enterocolitica has been paid so much attention as a kind of foodborne pathogen.The pre-enrichment of Yersinia enterocolitica was conducted by using the cultivation in TSB,PSB and ITC media.The pre-enrichment effect was evaluated by real-time PCR.The results indicated that the cultivation in TSB medium was better than other two media for pure Yersinia enterocolitica system.The enrichment time was 24 h.In addition,an enhanced sensitivity and shorten enrichment time（8 h） of Yersinia enterocolitica were achieved in the mixed microbe system containing TSB and ITC.Therefore,PSB can be used in the detection of Yersinia Enterocolitica in samples.