中文摘要：

目的探讨超声微泡造影剂介导色素上皮源性因子（PEDF）质粒转染大鼠视网膜、脉络膜的效率及治疗脉络膜新生血管（CNV）效果。方法氩绿激光对Long-Evans大鼠视网膜进行光凝建造CNV模型。将24只造模成功的CNV大鼠分为2组：①空白组,②超声辐照微泡转染组。于转染后14d,分别进行眼底荧光血管造影（FFA）,RT-PCR和免疫荧光检查。结果转染后14天超声微泡能介导PEDF质粒对大鼠视网膜、脉络膜的转染,并且对CNV有抑制作用。结论利用一定能量的超声击碎携带PEDF质粒的超声微泡造影剂,能够有效地提高PEDF质粒在大鼠视网膜、脉络膜的转染效率,对大鼠脉络膜新生血管有一定抑制作用。

英文摘要：

Objective To investigate whether ultrasound-mediated microbubble destruction could effectively deliver PEDF gene to the rat retina and inhibit rat CNV.Methods Twenty-four CNV Long-Evans rats induced by argon laser were randomly divided into two groups：group 1,the rats were accepted no treatment;group 2,microbubbles attached with the naked plasmid DNA of PEDF were infused into the femoral vein of rats with exposed to ultrasound immediately.Rats were killed and eyes were enucleated at 14 days after treatment.Gene and protein expression of PEDF in the rats＇ retina and choroids was detected by RT-PCR and immunofluorescence respectively.The effect of PEDF gene transfer on CNV was examined by FFA.Results Fourteen days later,infection efficiency of the group 2 was higher than that of group 1（P〈0.05）.With the administration of ultrasound-mediated microbubbles destruction,the CNV were inhibited effectively.Conclusion The PEDF expression in rats＇ retina and chorioid was increased due to the administration of ultrasound-mediated microbubbles destruction resulted in CNV being inhibited.