中文摘要：

目的制备一种可作为基因载体的脂质超声造影剂，对其理化性质和兔肾显影效果及载基因的能力进行研究。 方法采用机械振荡法制备一种可作为基因载体脂质超声微泡造影剂，观察60^Coγ射线灭菌前后造影剂外观、形态、浓度、平均粒径和电位改变，并观察其对正常兔肾显影效果，检测其结合基因的能力。 结果自制脂质微泡的平均粒径范围为2．11～6．43μm，平均粒径2．79μm，粒径分布均匀，浓度约为（3．16±0．29）×10^9／ml；经60^Coγ射线灭菌后在显微镜下观察微泡形态，粒径大小无明显变化；体内造影显示该造影剂能够有效增强兔肾实质回声；基因结合量效优化结果显示，造影剂的最大基因结合的效率为22％，最大基因结合量为0．45μg／ml。 结论自制脂质超声造影剂符合理想超声造影剂的要求，性质稳定，制备简易，载基因效率高，可望成为一种新型的超声微泡造影剂以及基因或药物治疗的载体。

英文摘要：

Objective To prepare a kind of self-made lipid ultrasound microbubble contrast agent, to study its characteristics and the gene pVEGF loading ability in vitro and to assess its enhancement effects in contrast imaging on normal rabbit kidneys. Methods A kind of lipid microbubbles was prepared, and its appearance, concentration, mean diameter and potential were detected before and after irradiance of 60^Co γ Ray, and the gene loading ability of the microbubble was detected. Seven normal rabbits were examined to observe the enhancement time and intensity in the kidney parenchyma. Results The mean diameter range of the self-made lipid microbubbles was 2.11 6.43μm, the mean diameter was 2.79 μm and the concentration was （3.16±0. 29） × 10^9/ml. Those indices were not changed significantly after sterilization with irradiance of 60^Co γ Ray. The maximum ratio and amount of loading gene of microbubbles was 22 % and 0.45μg/ml respectively. The appearance and diameter of microbubbles were not changed obviously after gene loading. Contrast imaging showed that the self-made lipid microbubbles could enhance the intensity of gray-scale （GS） Conclusions The self-made lipid microbubbles are consistent to the standard of ideal ultrasound contrast agent, which may become a new ultrasound contrast agent and a vehicle for encapsulating with genes or drugs.