目的:建立以反相高效液相色谱法测定不同品种淫羊藿药材中5种主要黄酮类成分含量的方法。方法:色谱柱为Zorbax SB-C18(250mm×4.6mm,5μm),流动相为乙腈-水(梯度洗脱),检测波长为270nm,柱温为30℃,同时测定淫羊藿药材中淫羊藿苷、朝藿定A、朝藿定B、朝藿定C、宝藿苷Ⅰ的含量。结果:5种成分均能达到基线分离,线性良好。不同品种淫羊藿药材中5种主要黄酮类成分的含量差别较大。结论:建议在考察淫羊藿药材的质量时,不能只考察淫羊藿苷,应建立多种黄酮类成分测定指标,以全面控制淫羊藿药材的质量。
OBJECTIVE: To determine five main Flavonoids (Icariin, Epimedin A, Epimedin B, Epimedin C, Baohuoside Ⅰ) simultaneously in Herba Epimedii from different species by RP- HPLC. METHODS: The samples were separated on Zor- bax SB-C18(250 mm × 4.6 mm, 5μm) with mobile phase consisted of acetonitrile-water (gradient elution) . The detection wavelength was 270 nm and the column temperature was 30 ℃ .The contents of Icariin, Epimedin A, Epimedin B, Epimedin C, Baohuoside Ⅰ in Herba Epimedii were determined simultaneously. RESULTS: All the five Flavonoids reached baseline separation with good linearity. The contents of the five Flavonoids from different species varied significantly. CONCLUSION: The results suggest we should establish multiple indicators of Flavonoids instead of considering the index of Icariin only to achieve a full control on the quality of Herba Epimdii.