目的:建立补肾活血方中人参总皂苷含量测定方法。方法:以人参皂苷Re为对照品,香草醛-高氯酸显色,采用分光光度法于544 nm处测定补肾活血方中人参总皂苷的含量。结果:在50.1-150.3μg范围内,对照品Re的吸光度与含量线性关系良好(r=0.9996),平均回收率为99.95%,RSD=2.33%(n=6)。结论:该法精密度、重现性良好,可作为补肾活血方人参总皂苷的含量测定方法。
Objective: To establish a method for the content determination of ginsenosides in Bushen Huoxue prescription. Method: With ginsenoside Re as reference substance, the content of ginsenosides in Bushen Huoxue prescription was determined by spectrophotometry after HC104-vanilin staining. Result: The calibration curve of ginsenoside Re was linear in the range of 50.1μg -150.3μg. The average recovery was 99.95% and RSD was 2.33% (n=6). Conclusion: This method can be used for the quality control of Bushen Huoxue prescription with good precision and reproducibility.