中文摘要：

目的：建立准确快速测定大鼠口服葛根后血浆中大豆苷元含量的方法,探讨大豆苷元的体内代谢情况。方法：采用反相高效液相色谱法（RP-HPLC）测定大鼠口服药物后不同时间血浆中大豆苷元的含量,待测血浆样品采用沉淀蛋白法处理,Phenomenex Luna C18高效液相色谱柱（4.60 mm×250 mm,5μm）进行分析,流动相为甲醇-0.1%甲酸水（60：40v/v）。结果：测定方法稳定,给药后5 min血浆中即可检测出大豆苷元,20 min内达到峰值,高、中、中低、低4种浓度的日内精密度均≤5.7%,日间精密度均≤9.3%,回收率分别为89.5%、93.2%、93.9%、84.8%,在0.1～64μg/mL范围内线性关系良好。结论：此方法快捷、灵敏、简便,用于血浆中大豆苷元的含量测定,取得满意结果。

英文摘要：

AIM：To explore a RP-HPLC method for determining daidzein content of rat plasma after oral administration of Pueraria lobata.METHODS：The analysis was performed on a Phenomenex Luna C18 column（4.60mm×250 mm,5 μm） with the mobile phase consisted of CH3OH and 0.1% aqueous formic acid（60：40,v/v）.The detection wavelength was set at 260 nm.RESULTS：In five minutes after the oral administration,daidzein was detected in rat plasma,and the plasma level reached the peak value within 20 minutes.The calibration curve was linear in the range of 0.1-64 μg/mL.The intraday and inter precisions were less than 5.70% and 9.3%,respectively.The recovery rate of four different concentrations was 89.5%,93.2%,93.9% and 84.8%,respectively.CONCLUSION：In this paper,RP-HPLC proves to be a suitable method for determining the plasma levelof daidzein.