中文摘要：

目的：探讨铅暴露工人外周血淋巴细胞遗传损伤情况与端粒长度改变之间的关系。方法：以珠三角某蓄电池厂的21名铅暴露工人为铅暴露组,26名无铅接触史的工人作为对照组。针对工人开展问卷调查并采集肝素抗凝外周血。检测工人外周血中血铅浓度、淋巴细胞的彗星试验指标、胞质分裂阻滞微核试验指标以及相对端粒长度改变情况。结果：铅暴露工人的血铅浓度为（3.08±1.86）mg/mL,与对照人群（0.26±0.25）mg/mL相比明显升高（P〈0.05）。彗星试验中,铅暴露组工人的慧星尾长高于对照组（P〈0.05）;胞质分裂阻滞微核试验中,暴露组工人双核微核率和微核细胞率均高于对照组（P〈0.05）;铅暴露工人的外周血相对端粒长度较对照人群缩短（P〈0.05）。相关分析结果显示,胞质阻滞微核试验的指标中双核微核率、微核细胞率、核桥率与工龄呈正相关（rs分别为0.447、0.432、0.351,P均〈0.05）。外周血相对端粒长度与工人工龄、血铅水平均呈负相关（r_s分别为-0.306、-0.394,（P均〈0.05）。结论：铅暴露可致工人外周血淋巴细胞的遗传损伤以及相对端粒长度的缩短。

英文摘要：

OBJECTIVE：To investigate the induction of DNA damage and telomere length changes in peripheral leukocytes from lead exposed works in comparison with un-exposed controls.METHODS：21lead exposed workers from a battery plant in the Pearl River Delta Region and 26 non-exposed workers were investigated.Questionnaires were surveyed.Blood samples and peripheral leukocytes were analyzed for blood lead concentrations,telomere length,Comet assay,and cytokinesis-block micronucleus assay.RESULTS：Blood lead concentration of exposed workers（3.08 ± 1.86）mg/mL was significantly higher than that（0.26 ± 0.25） mg/mL of the controls（P〈0.05）.In addition,tail lengthsand micronuclei frequencies in leukocytes from the exposed workers were significantly higher than that of the controls（P〈0.05）.However,relative telomere lengths from leukocytes of lead exposed workers were shorter than that of the controls（P〈0.05）.Correlation analyses indicated that micronucleus frequencies in binucleated cells,rate of micronucleated binucleate cells and rate of nucleoplasmic bridges were positively correlated with work age（P〈0.05）,and the rank correlation coefficient（r_s） were 0.447,0.432,0.351 respectively.Work age and blood lead concentration were negatively correlated with relative telomere length of leukocytes（P0.05）,and r_s were-0.306,-0.394 respectively.CONCLUSION：Lead exposure induced DNA damage,micronuclei and shorter telomere length in peripheral leukocytes of exposed workers.