中文摘要：

目的：建立CCM3基因敲降的斑马鱼模型，探讨其作为水体中低浓度心血管毒性污染物的生物监测模型的可能性。方法：选取绿色荧光标记的血管内皮细胞生长因子受体2(VEGFR2)转基因斑马鱼作为研究对象，采用吗啡啉修饰的反义寡聚核苷酸(MO)对斑马鱼单细胞期受精卵进行显微注射，采用激光共聚焦显微镜观察受精后72h的幼鱼脑部血管发育形态，建立CCM3基因敲降的斑马鱼模型。通过改进寇氏法测定铅对斑马鱼的半数致死剂量(LD)。在低浓度10mg/L铅暴露下分别对正常基因型和50CCM3基因敲降型的斑马鱼卵进行染毒，同时设立对照组(无铅暴露)，观察各组斑马鱼发育情况。结果：野生型斑马鱼铅染毒的LD为31.24mg/L。在相同低浓度10mg/L铅暴露的情况下，受精后72h时正常基因型的斑马鱼幼鱼和对照组的斑马鱼幼鱼各项发育50指标无明显异常，而注射亚表型剂量的CCM3MO0.25ng组斑马幼鱼出现形态发育异常表型，如发育迟缓、脊柱弯曲、心包水肿、卵黄囊水肿等。结论：CCM3基因敲降的斑马鱼模型可作为水体中具有心血管毒性污染物的敏感生物监测模型。

英文摘要：

OBJECTIVE:To establishment a zebrafish model with CCM3gene knockdown and to investigate its usefulness as a biological monitoring model for low concentrations of cardiovascular toxicants in water.METHODS:The transgenic zebrafish line(VEGFR2:GFP)which expresses green flurescence proteins in blood vessels were used.The one-cell stage zebrafish eggs were injected with morpholino oligos(MO)at indicated doses.Laser scanning confocal microscopy was used to observe the intracranial vessels of zebrafish at72h.The zebrafish model with CCM3gene knockdown was established.Karber’s method was used to calculate the median lethal dose(LD)of lead for zebrafish.The50wild-type and CCM3gene knockdown zebrafish were exposed to low concentrations of lead(10mg/L).Development of zebrafish of each group was documented.RESULTS:The LD value for wild-type zebrafish was31.24mg/L.Zebrafish50with CCM3gene knockdownt were more susceptible to low concentrations of lead-exposure(10mg/L)than the wild-type by displaying abnormal phenotype,such as decreased body length,pericardial edema,hemorrhage,egg yolk edema,spine distortions.Up to72h,after treatment with the low dose,the wild-type zebrafish did not show any abnormal phenotype.CONCLUSION:The CCM3gene knockdown zebrafish can possibly be used as a sensitive biological monitoring model for cardiovascular toxicants in water.