由小麦品种花培3号和豫麦57杂交获得了168个株系的DH群体为材料,分别用蒸馏水(对照)以及50、100mmol/L Na Cl溶液处理,对小麦幼苗的苗高、苗干重进行了QTL定位及效应分析。利用完备区间作图法,共检测到16个加性QTL和17对上位性互作QTL。其中,检测到8个控制苗高的QTL,分布在小麦2A、2D、3B、4D、6B和7B染色体上,单个QTL可解释3.38%-22.96%的遗传变异,位于4D和7B染色体上控制苗高的QSH4D和QSH7B两个QTL位点在两个环境中均被检测到,QSH4D在两个环境里的遗传贡献率分别为17.9%和22.96%,为一主效QTL位点;检测到8个控制苗干重的QTL,分布在小麦1A、1B、2B、2D、4D和5B染色体上,单个QTL可解释4.53%-9.10%的遗传变异。在1A染色体上控制小麦幼苗苗干重的QDSW1A,在盐胁迫和非盐胁迫下均稳定表达,贡献率分别为7.78%和7.87%,可用于小麦耐盐的分子标记辅助选择。加性效应和上位效性均对小麦幼苗苗高和苗干重的遗传起重要作用。
Seedling height and dry seedling weight are important indexes to evaluate salt tolerance of wheat seedlings. To detect quantitative trait loci(QTL) associated with seedling height and dry seedling weight at seedling stage in wheat, a set of168 doubled haploid(DH) lines derived from the cross between Huapei 3 and Yumai 57 was treated with distilled water(CK),50 and 100 mmol /L of Na Cl. Based on inclusive composite interval mapping(ICIM) method, we identified 16 additive QTLs and 17 pairs of epistatic QTLs for seedling height and dry seedling weight under CK and salt stress. A total of eight QTLs for seedling height were detected distributed on chromosomes 2A, 2D, 3B, 4D, 6B and 7B, and explained phenotypic variation ranging from 3.38 % to 22.96 %. A total of eight QTLs for dry seedling weight were detected distributed on chromosomes 1A,1B, 2B, 2D, 4D and 5B, which accounted for 4.53-9.10 % of the phenotypic variation.The QDSW1 A for dry seedling weight expressed both in CK and salt stress, which could be used in marker-assisted selection in wheat breeding programs. The results indicate that both additive effects and epistatic effects are important genetic bases for wheat seedling traits.