为了给小麦营养品质改良提供依据,以花培3号/豫麦57构建的小麦DH群体的168个株系为材料,利用该群体两年的表型数据,对小麦籽粒所含17种氨基酸、必需氨基酸、非必需氨基酸及氨基酸总量的遗传变异及其之间的相关性进行了分析。结果表明,小麦籽粒氨基酸含量组成不平衡,谷氨酸在籽粒总氨基酸中所占比例最高,达25.81%;亮氨酸和脯氨酸次之;赖氨酸和组氨酸在籽粒总氨基酸中所占比例最低,仅2.27%和2.10%。所有氨基酸含量在两个年份中的变异系数均较大(大于7%),半胱氨酸含量在两个环境中的平均变异系数最大,为23.16%;天冬氨酸含量的平均变异系数最小,为7.62%。除赖氨酸和半胱氨酸含量在两个年份中均不存在相关性外,其余氨基酸含量之间显著或极显著正相关,多数氨基酸含量在两个年份中均存在极显著的正相关;同时两年数据略有差异,说明小麦籽粒氨基酸含量受气候条件影响。
To enhance amino acids content and improve nutritional quality in wheat, a DH population derived from the cross between Huapei 3 and Yumai 57 was used to investigate the genetic variation and correlation of 17 amino acids of essential amino acid, non essential amino acid and total amino acid using the data from two years' experiment. The results showed that amino acid composition was imbalanced in wheat kernel, glutamic acid shared the highest proportion of the total amino acid, which was 25.81%, followed by leucine and proline, and the proportions for lysine and histidine were the lowest, which was 2.27% and 2.10%, respectively. All amino acids had high coefficient of variation (more than 7%) in the two years, cysteine, methionine and proline had the highest coefficient of variation, which were 23.16%, 22.88% and 20.30%, respectively, and aspartic acid, glycine and lysine had the lowest coefficient of variation, which were 7.62%, 8.29% and 8.62%, respectively. Correlation analysis showed that there was no correlation between lysine and cysteine in the two years, while there were significant or highly significant and positive correlations between the remaining amino acids. And there was slight difference between the amino acid contents obtained from the two years, which indicated that amino acids contents of wheat were affected by weather conditions.