中文摘要：

【目的】木质纤维素是世界上储量最丰富、最廉价的可再生生物质资源,以米根霉为研究对象,探讨对木质纤维素中主要单糖成分——木糖和葡萄糖的代谢差异,为木质纤维素的高效利用提供科学依据。【方法】分别以木糖和葡萄糖为碳源,考察米根霉的生物量、细胞大分子组分、胞内还原力（NADH/NAD＋）、ATP含量以及有机酸积累的差异。【结果】以木糖为碳源时,米根霉生物量较高,达到9.93 g/L,对糖转化率达37.2%,同时胞内还原力（NADH/NAD＋）和ATP含量较高,几乎不积累有机酸;以葡萄糖为碳源时,米根霉生物量为7.28 g/L,对糖转化率仅25.46%,细胞大分子组分、胞内还原力以及ATP的含量较低,但有机酸（乳酸为主）积累量较高。在混糖条件下培养时,米根霉优先利用葡萄糖,当剩余葡萄糖浓度较低时,转向利用木糖。【结论】米根霉代谢木糖和葡萄糖时,胞内还原力和ATP等能量代谢存在较大差异,前者趋向生物大分子合成和生物量的积累,后者趋向积累有机酸等小分子。

英文摘要：

[Objective]As one of the most abandon and cheapest recyclable bio-resources,cellulosic biomass appear to be the promising new energy if the two major components,glucose and xylose,are efficiently used.[Methods] We compared the metabolism of Rhizopus oryzae growing on glucose or xylose.We measured biomass accumulation,intercellular metabolite contents,and organic acids production.[Results]When cultured on xylose,the rate of bio-transformation reached 35.2% compared to 24.3% in the glucose.Intercellular contents accumulated much more by using xylose as sole carbon than using glucose.However,the quantity of organic acid was less with xylose than with glucose.In addition,during the coexistence of both substrates,Rhizopus oryzae utilizes glucose first,and xylose later when the glucose is almost exhausted.[Conclusion] With our data analysis,both the NADH /NAD ＋ and ATP quantity are differed between the utilization of two carbon resources.In xylose,biological macromolecule and biomass accumulation are in advantage,while in glucose,the inorganic acid production is enhanced.