中文摘要：

针对米根霉发酵产富马酸使用的不同中和剂（CaCO3，Na2CO3，NH3·H2O,NaOH）进行了研究，结果表明发酵过程中使用Na2CO3作为中和剂时富马酸产率和生产强度最接近传统中和剂CaCO3。此后考察了不同pH值（3．5，4．5，5．5和6．5）对Na2CO3作为中和剂的富马酸发酵过程的影响。基于对3个动力学参数的分析，提出了一个旨在同时获得富马酸高产物浓度、高产率和高生产强度的双阶段pH调控策略，在初始的24h内pH控制在5．5，然后将pH调到4．5直至发酵结束。最终富马酸的终浓度达到40．5g／L，产率为0．55g／g，生产强度为0．61g／L／h，比恒定pH时的最优结果分别提高了8．3％，10．0％和17．3％，其中生产强度甚至比使用CaCO3时还高了3．4％。故以Na2CO3作为中和剂，采用双阶段pH调控策略具有降低能耗和简化下游步骤的优势，可以成功取代CaCO，。

英文摘要：

Four different neutralizing agents （CaCO3, Na2C03, NH3· H2O, NaOH） were chosen to examine the effects of neutralizing agents on fumaric acid fermentation by Rhizopus oryzae ME-F12 which is the mutant of R. oryzae ATCC 20344. It was found that fumaric acid yield and productivity in the fermentation using Na2 CO3 as neutralizing agent were the closest to which in the traditional CaCO3 case. Then the effects of different pH values （3.5, 4.5, 5.5, and 6.5） on fumaric acid fermentation using Na2CO3 as neutralizing agent were investigated. Based on the analysis of three kinetic parameters, a two-stage pH control strategy, aimed at achieving high concentration, high yield and high productivity of fumaric acid simultaneously, was proposed, pH was controlled at 5.5 at the first 24 h, and then switched to d. 5 till the end of the fermentation. Finally, the maximum concentration of fumaric acid reached 40.5 g/L with the yield of 0.55 g/g and the productivity of 0.61 g/L/h, which were 8.3%, 10.0% and 17.3% higher than the best result of constant pH control process. Its productivity was even 3.4% higher than which in CaCO3 case. With the internal advantages of reducing power consumption and simplifying downstream processing, using Na2CO3 as neutralizing agent under two-stage pH control strategy successfully take place of CaCO3