中文摘要：

以膜反应器固定化米根霉发酵产富马酸为研究对象,以Na2CO3为中和剂,考察固定化米根霉在5L搅拌式发酵罐中的发酵特征,采用智能可视化软件（IVOS）优化发酵工艺条件.结果表明,在80g/L初始糖浓及最优工艺下,富马酸产量、生产速率及转化率分别为21.1g/L、0.25g/ （L-h）和28％;采用40g/L初始糖浓及连续批次发酵工艺时,富马酸产量、生产速率及转化率最高分别为10.8 g/L、0.36g/（L＆#183;h）和27％.搅拌式反应器中,固定化米根霉的膜反应器比表面积有限,以及菌膜的空间阻隔效应对传质传氧的限制作用,显著影响了富马酸的生产强度和转化率.因此,亟需发掘新的固定化方法及反应器形式,达到既解决米根霉形态控制问题,又有助于生产性状提升的目标.

英文摘要：

The production of fumaric acid was optimized using immobilized Rhizopus oryzae with biofilm reactor and neutralized with Na2CO3 in 5L stirred tank reactor. Visualization method （IVOS） was used to optimize the process for fumaric acid production. Under the optimized condition （80g/L total glucose）, the best results were fumaric acid titer of 21.10g/L, the volumetric productivity of 0.25 g/L ~ h and the yield of 28% , respectively; in the continuous batch fermentation condition（4Og/L total glucose）, the best results were fumaric acid titer of 10.8 g/L,yield and volumetric productivity of fumarate were 27% and 0.36 g/L · h, respectively. Owing to the limitation of specific surface area of biofilm and its blocking mass transfer, biofilm reactor could not give an effective fumaric acid production. Future prospects for improvement of fumaric acid production include developing new methods for immobilized cell and bioreactor.