中文摘要：

目的应用530 nm单色光光照建立一种新型近视眼动物模型。方法20只约2周龄健康雄性豚鼠,随机分成两组（n=10）,实验组和对照组分别在绿光（530 nm）和白光（色温5000 k）下进行饲养。设置照明参数：光量子数相同,为每秒3×10-4μmol/cm2;实测光强度绿光为0.150 mW/cm2,白光为0.247 mW/cm2。实验前每组进行眼球生物学测量（屈光度、角膜曲率、眼轴各部分长度）,光照后12周重复测量以上数据,每只豚鼠均取右侧眼参数进行统计分析。结果光照前两组生物学测量参数差异无显著性。光照12周后,绿光组屈光度发生-3.125±0.76 D的变化,白光组为-1.075±0.71D,绿光组同对照白光组相比平均形成约2.0 D的近视,差异有显著性;绿光组眼轴和玻璃体腔分别增长0.98±0.13 mm与0.33±0.14 mm,对照组分别为0.77±0.22 mm与0.13±0.14 mm,绿光组较对照组眼轴和玻璃体腔长度延长较快,差异有显著性;光照后两组角膜曲率半径、前房深度和晶状体厚度均发生不同程度增加,但两组间变化差异无显著性。结论530 nm单色光诱导豚鼠眼球眼轴和玻璃体腔长度延长较快,产生近视.

英文摘要：

Objective In the early life period when eyes grow rapidly,visual experience can play an important role in axial growth and refractive development.For instance,depriving the eye of form vision during infancy will accelerate axial growth,resulting in substantial amounts of myopia,called form deprivation myopia（FDM）.Similarly,imposing the eye with a negative lens produces compensating myopic growth in many species,called defocus induced myopia（DIM）.As one of the important visual experiences,color vision and its effects on eye growth deserve to be investigated.The purpose of this study was to investigate the effect of 530 nm monochromatic light and establish an innovative model of myopia in guinea pig by exposing to this monochromatic light.Methods Twenty male guinea pigs at 2 weeks old were randomly assigned to two groups（n=10）.The experimental group was raised under the condition of 530 nm monochromatic light illumination.The control one was bred under white light illumination with 5000 k color temperature. These guinea pigs were raised in a specially designed cage.The light source was provided by specially made LEDs（green： peak value 530 nm and half bandwidth 30 nm;white： color temperature 5000 K）.The illumination parameters of the two groups were identical and the light quantum number was 3×10~（-4）μmol·cm~（-2）·s~（-1）.Through measuring,the irradiance value was 0.150 mW·cm~（-2） for green light and 0.247 mW·cm~（-2） for white light approximately.All animals were kept under a 12/12 h light/dark cycle（light： 8 a.m.-8 p.m.）,in the temperature of 22℃-26℃ and a relative humidity of 55%-65%.Both groups underwent biometric measurement including refraction,corneal curvature and axial length,etc.before and after twelve weeks treatment.The refraction was examined using a streak retinoscope and trial lenses in a dark room one hour after topically administering a cycloplegic eye drop.The radius of corneal curvature was measured with a keratometer（Topcon OM-4,Japan） and